Soluble receptor for advanced glycation end products (sRAGE) is present at high concentrations in the lungs of children and varies with age and the pattern of lung inflammation

Stephanie Yerkovich; Anne Chang; Melanie Carroll; Helen Petsky; Greta Scrivener; John Upham

doi:10.1111/j.1440-1843.2012.02174.x

Soluble receptor for advanced glycation end products (sRAGE) is present at high concentrations in the lungs of children and varies with age and the pattern of lung inflammation

Stephanie Yerkovich, Anne Chang, Melanie Carroll, Helen Petsky, Greta Scrivener, John Upham

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Abstract

Background and objective: The soluble receptor for advanced glycation end products (sRAGE) plays an important role in inflammation. Few studies have looked at sRAGE levels in human lungs, and there is no information in children. Therefore, this study aimed to compare bronchoalveolar lavage fluid (BALF) and plasma sRAGE concentrations in children in relation to age and inflammation.

Methods: BAL was performed in 76 children, and BALF and plasma sRAGE levels were determined by enzyme-linked immunosorbent assay.

Results: sRAGE levels were fourfold higher in BALF than in plasma (P < 0.001). BALF sRAGE was inversely proportional to age (r = −0.333, P = 0.008) and serum immunoglobulin A (r = −0.283, P = 0.028). Plasma sRAGE showed a positive correlation to the percentage of BAL macrophages and negative correlation to the percentage of neutrophils and lymphocytes (P < 0.05). Multivariate linear regression analysis identified that the percentage of BAL lymphocytes and neutrophils were significant independent predictors of plasma sRAGE levels, while age and the percentage of BAL macrophages independently predicted BALF sRAGE levels.

Conclusions: In children, sRAGE is present at higher concentrations in the lung compared with blood. It appears that sRAGE varies with age, and hence future studies of sRAGE in paediatric lung disease require age matching. The significant relationship between sRAGE and lung inflammation warrants further research.

Original language	English
Pages (from-to)	841-846
Number of pages	6
Journal	Respirology
Volume	17
Issue number	5
DOIs	https://doi.org/10.1111/j.1440-1843.2012.02174.x
Publication status	Published - Jul 2012

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@article{cbcf695547f94cf4ba95d6d3ecb30b87,

title = "Soluble receptor for advanced glycation end products (sRAGE) is present at high concentrations in the lungs of children and varies with age and the pattern of lung inflammation",

abstract = "Background and objective: The soluble receptor for advanced glycation end products (sRAGE) plays an important role in inflammation. Few studies have looked at sRAGE levels in human lungs, and there is no information in children. Therefore, this study aimed to compare bronchoalveolar lavage fluid (BALF) and plasma sRAGE concentrations in children in relation to age and inflammation.Methods: BAL was performed in 76 children, and BALF and plasma sRAGE levels were determined by enzyme-linked immunosorbent assay.Results: sRAGE levels were fourfold higher in BALF than in plasma (P < 0.001). BALF sRAGE was inversely proportional to age (r = −0.333, P = 0.008) and serum immunoglobulin A (r = −0.283, P = 0.028). Plasma sRAGE showed a positive correlation to the percentage of BAL macrophages and negative correlation to the percentage of neutrophils and lymphocytes (P < 0.05). Multivariate linear regression analysis identified that the percentage of BAL lymphocytes and neutrophils were significant independent predictors of plasma sRAGE levels, while age and the percentage of BAL macrophages independently predicted BALF sRAGE levels.Conclusions: In children, sRAGE is present at higher concentrations in the lung compared with blood. It appears that sRAGE varies with age, and hence future studies of sRAGE in paediatric lung disease require age matching. The significant relationship between sRAGE and lung inflammation warrants further research.",

keywords = "advanced glycation end product receptor, immunoglobulin A, immunoglobulin G, immunoglobulin M, age, article, child, enzyme linked immunosorbent assay, female, human, human cell, humoral immunity, immunoglobulin blood level, infant, lung, lung alveolus macrophage, lung lavage, lymphocyte count, major clinical study, male, neutrophil count, pneumonia, preschool child, priority journal, protein blood level, tissue level, Adolescent, Adult, Aging, Bronchoalveolar Lavage Fluid, Bronchoscopy, Child, Female, Humans, Immunity, Humoral, Immunoglobulin A, Lung, Male, Multivariate Analysis, Pneumonia, Receptors, Immunologic, Retrospective Studies, Young Adult",

author = "Stephanie Yerkovich and Anne Chang and Melanie Carroll and Helen Petsky and Greta Scrivener and John Upham",

year = "2012",

month = jul,

doi = "10.1111/j.1440-1843.2012.02174.x",

language = "English",

volume = "17",

pages = "841--846",

journal = "Respirology",

issn = "1323-7799",

publisher = "Wiley-Blackwell",

number = "5",

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Soluble receptor for advanced glycation end products (sRAGE) is present at high concentrations in the lungs of children and varies with age and the pattern of lung inflammation. / Yerkovich, Stephanie; Chang, Anne; Carroll, Melanie; Petsky, Helen; Scrivener, Greta; Upham, John.

In: Respirology, Vol. 17, No. 5, 07.2012, p. 841-846.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Soluble receptor for advanced glycation end products (sRAGE) is present at high concentrations in the lungs of children and varies with age and the pattern of lung inflammation

AU - Yerkovich, Stephanie

AU - Chang, Anne

AU - Carroll, Melanie

AU - Petsky, Helen

AU - Scrivener, Greta

AU - Upham, John

PY - 2012/7

Y1 - 2012/7

N2 - Background and objective: The soluble receptor for advanced glycation end products (sRAGE) plays an important role in inflammation. Few studies have looked at sRAGE levels in human lungs, and there is no information in children. Therefore, this study aimed to compare bronchoalveolar lavage fluid (BALF) and plasma sRAGE concentrations in children in relation to age and inflammation.Methods: BAL was performed in 76 children, and BALF and plasma sRAGE levels were determined by enzyme-linked immunosorbent assay.Results: sRAGE levels were fourfold higher in BALF than in plasma (P < 0.001). BALF sRAGE was inversely proportional to age (r = −0.333, P = 0.008) and serum immunoglobulin A (r = −0.283, P = 0.028). Plasma sRAGE showed a positive correlation to the percentage of BAL macrophages and negative correlation to the percentage of neutrophils and lymphocytes (P < 0.05). Multivariate linear regression analysis identified that the percentage of BAL lymphocytes and neutrophils were significant independent predictors of plasma sRAGE levels, while age and the percentage of BAL macrophages independently predicted BALF sRAGE levels.Conclusions: In children, sRAGE is present at higher concentrations in the lung compared with blood. It appears that sRAGE varies with age, and hence future studies of sRAGE in paediatric lung disease require age matching. The significant relationship between sRAGE and lung inflammation warrants further research.

AB - Background and objective: The soluble receptor for advanced glycation end products (sRAGE) plays an important role in inflammation. Few studies have looked at sRAGE levels in human lungs, and there is no information in children. Therefore, this study aimed to compare bronchoalveolar lavage fluid (BALF) and plasma sRAGE concentrations in children in relation to age and inflammation.Methods: BAL was performed in 76 children, and BALF and plasma sRAGE levels were determined by enzyme-linked immunosorbent assay.Results: sRAGE levels were fourfold higher in BALF than in plasma (P < 0.001). BALF sRAGE was inversely proportional to age (r = −0.333, P = 0.008) and serum immunoglobulin A (r = −0.283, P = 0.028). Plasma sRAGE showed a positive correlation to the percentage of BAL macrophages and negative correlation to the percentage of neutrophils and lymphocytes (P < 0.05). Multivariate linear regression analysis identified that the percentage of BAL lymphocytes and neutrophils were significant independent predictors of plasma sRAGE levels, while age and the percentage of BAL macrophages independently predicted BALF sRAGE levels.Conclusions: In children, sRAGE is present at higher concentrations in the lung compared with blood. It appears that sRAGE varies with age, and hence future studies of sRAGE in paediatric lung disease require age matching. The significant relationship between sRAGE and lung inflammation warrants further research.

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KW - immunoglobulin G

KW - immunoglobulin M

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KW - female

KW - human

KW - human cell

KW - humoral immunity

KW - immunoglobulin blood level

KW - infant

KW - lung

KW - lung alveolus macrophage

KW - lung lavage

KW - lymphocyte count

KW - major clinical study

KW - male

KW - neutrophil count

KW - pneumonia

KW - preschool child

KW - priority journal

KW - protein blood level

KW - tissue level

KW - Adolescent

KW - Adult

KW - Aging

KW - Bronchoalveolar Lavage Fluid

KW - Bronchoscopy

KW - Child

KW - Female

KW - Humans

KW - Immunity, Humoral

KW - Immunoglobulin A

KW - Lung

KW - Male

KW - Multivariate Analysis

KW - Pneumonia

KW - Receptors, Immunologic

KW - Retrospective Studies

KW - Young Adult

U2 - 10.1111/j.1440-1843.2012.02174.x

DO - 10.1111/j.1440-1843.2012.02174.x

M3 - Article

VL - 17

SP - 841

EP - 846

JO - Respirology

JF - Respirology

SN - 1323-7799

IS - 5

ER -

Soluble receptor for advanced glycation end products (sRAGE) is present at high concentrations in the lungs of children and varies with age and the pattern of lung inflammation

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