Spectrophotometry assays to determine G6PD activity from Trinity Biotech and Pointe Scientific G6PD show good correlation

Mohammad Shafiul Alam, Mohammad Golam Kibria, Nusrat Jahan, Ric N. Price, Benedikt Ley

    Research output: Contribution to journalComment/debateResearchpeer-review

    Abstract

    Objectives: Spectrophotometry kits from Pointe Scientific (PS; USA) were compared to kits from Trinity Biotech (Trinity; Ireland) in 50 venous blood samples from purposively selected individuals in Bangladesh. Repeatability and inter-assay variability were assessed by Students t-test, Bland-Altman plot and Pearson correlation coefficient (r). The median glucose-6-phosphate dehydrogenase (G6PD) activity of all G6PD normal participants was calculated per assay and defined as 100% activity. Performance was calculated considering 30% and 70% cut off activities and Trinity as reference.

    Results: The intra-assay correlation of Trinity (r = 0.9841, p < 0.001) and PS (r = 0.9833, p < 0.001) did not differ significantly (p = 0.904). Both assays were closely correlated (r = 0.9799, p < 0.001), with a mean difference of 0.1 U/gHb (95% limit of agreement: - 1.32 to 1.57). At 30% cut off PS had a sensitivity of 100% (95% confidence interval (95 CI) 59.0-100.0) and specificity of 100% (95% CI 91.8 to 100.0), at 70% cut-off of 100% (95% CI 79.4-100.0) and 97.1% (95% CI 84.7-99.9) respectively. The G6PD assay from PS is a reliable alternative to the assay from Trinity.

    Original languageEnglish
    Article number855
    Pages (from-to)1-4
    Number of pages4
    JournalBMC Research Notes
    Volume11
    Issue number1
    DOIs
    Publication statusPublished - 4 Dec 2018

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    Glucosephosphate Dehydrogenase
    Spectrophotometry
    Assays
    Bangladesh
    Ireland
    Confidence Intervals
    Students
    Blood

    Cite this

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    title = "Spectrophotometry assays to determine G6PD activity from Trinity Biotech and Pointe Scientific G6PD show good correlation",
    abstract = "Objectives: Spectrophotometry kits from Pointe Scientific (PS; USA) were compared to kits from Trinity Biotech (Trinity; Ireland) in 50 venous blood samples from purposively selected individuals in Bangladesh. Repeatability and inter-assay variability were assessed by Students t-test, Bland-Altman plot and Pearson correlation coefficient (r). The median glucose-6-phosphate dehydrogenase (G6PD) activity of all G6PD normal participants was calculated per assay and defined as 100{\%} activity. Performance was calculated considering 30{\%} and 70{\%} cut off activities and Trinity as reference. Results: The intra-assay correlation of Trinity (r = 0.9841, p < 0.001) and PS (r = 0.9833, p < 0.001) did not differ significantly (p = 0.904). Both assays were closely correlated (r = 0.9799, p < 0.001), with a mean difference of 0.1 U/gHb (95{\%} limit of agreement: - 1.32 to 1.57). At 30{\%} cut off PS had a sensitivity of 100{\%} (95{\%} confidence interval (95 CI) 59.0-100.0) and specificity of 100{\%} (95{\%} CI 91.8 to 100.0), at 70{\%} cut-off of 100{\%} (95{\%} CI 79.4-100.0) and 97.1{\%} (95{\%} CI 84.7-99.9) respectively. The G6PD assay from PS is a reliable alternative to the assay from Trinity.",
    keywords = "G6PD, Malaria, Spectrophotometry",
    author = "Alam, {Mohammad Shafiul} and Kibria, {Mohammad Golam} and Nusrat Jahan and Price, {Ric N.} and Benedikt Ley",
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    month = "12",
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    Spectrophotometry assays to determine G6PD activity from Trinity Biotech and Pointe Scientific G6PD show good correlation. / Alam, Mohammad Shafiul; Kibria, Mohammad Golam; Jahan, Nusrat; Price, Ric N.; Ley, Benedikt.

    In: BMC Research Notes, Vol. 11, No. 1, 855, 04.12.2018, p. 1-4.

    Research output: Contribution to journalComment/debateResearchpeer-review

    TY - JOUR

    T1 - Spectrophotometry assays to determine G6PD activity from Trinity Biotech and Pointe Scientific G6PD show good correlation

    AU - Alam, Mohammad Shafiul

    AU - Kibria, Mohammad Golam

    AU - Jahan, Nusrat

    AU - Price, Ric N.

    AU - Ley, Benedikt

    PY - 2018/12/4

    Y1 - 2018/12/4

    N2 - Objectives: Spectrophotometry kits from Pointe Scientific (PS; USA) were compared to kits from Trinity Biotech (Trinity; Ireland) in 50 venous blood samples from purposively selected individuals in Bangladesh. Repeatability and inter-assay variability were assessed by Students t-test, Bland-Altman plot and Pearson correlation coefficient (r). The median glucose-6-phosphate dehydrogenase (G6PD) activity of all G6PD normal participants was calculated per assay and defined as 100% activity. Performance was calculated considering 30% and 70% cut off activities and Trinity as reference. Results: The intra-assay correlation of Trinity (r = 0.9841, p < 0.001) and PS (r = 0.9833, p < 0.001) did not differ significantly (p = 0.904). Both assays were closely correlated (r = 0.9799, p < 0.001), with a mean difference of 0.1 U/gHb (95% limit of agreement: - 1.32 to 1.57). At 30% cut off PS had a sensitivity of 100% (95% confidence interval (95 CI) 59.0-100.0) and specificity of 100% (95% CI 91.8 to 100.0), at 70% cut-off of 100% (95% CI 79.4-100.0) and 97.1% (95% CI 84.7-99.9) respectively. The G6PD assay from PS is a reliable alternative to the assay from Trinity.

    AB - Objectives: Spectrophotometry kits from Pointe Scientific (PS; USA) were compared to kits from Trinity Biotech (Trinity; Ireland) in 50 venous blood samples from purposively selected individuals in Bangladesh. Repeatability and inter-assay variability were assessed by Students t-test, Bland-Altman plot and Pearson correlation coefficient (r). The median glucose-6-phosphate dehydrogenase (G6PD) activity of all G6PD normal participants was calculated per assay and defined as 100% activity. Performance was calculated considering 30% and 70% cut off activities and Trinity as reference. Results: The intra-assay correlation of Trinity (r = 0.9841, p < 0.001) and PS (r = 0.9833, p < 0.001) did not differ significantly (p = 0.904). Both assays were closely correlated (r = 0.9799, p < 0.001), with a mean difference of 0.1 U/gHb (95% limit of agreement: - 1.32 to 1.57). At 30% cut off PS had a sensitivity of 100% (95% confidence interval (95 CI) 59.0-100.0) and specificity of 100% (95% CI 91.8 to 100.0), at 70% cut-off of 100% (95% CI 79.4-100.0) and 97.1% (95% CI 84.7-99.9) respectively. The G6PD assay from PS is a reliable alternative to the assay from Trinity.

    KW - G6PD

    KW - Malaria

    KW - Spectrophotometry

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    U2 - 10.1186/s13104-018-3964-7

    DO - 10.1186/s13104-018-3964-7

    M3 - Comment/debate

    VL - 11

    SP - 1

    EP - 4

    JO - BMC Research Notes

    JF - BMC Research Notes

    SN - 1756-0500

    IS - 1

    M1 - 855

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