Spectrophotometry assays to determine G6PD activity from Trinity Biotech and Pointe Scientific G6PD show good correlation

Mohammad Shafiul Alam, Mohammad Golam Kibria, Nusrat Jahan, Ric N. Price, Benedikt Ley

Research output: Contribution to journalComment/debateResearchpeer-review

Abstract

Objectives: Spectrophotometry kits from Pointe Scientific (PS; USA) were compared to kits from Trinity Biotech (Trinity; Ireland) in 50 venous blood samples from purposively selected individuals in Bangladesh. Repeatability and inter-assay variability were assessed by Students t-test, Bland-Altman plot and Pearson correlation coefficient (r). The median glucose-6-phosphate dehydrogenase (G6PD) activity of all G6PD normal participants was calculated per assay and defined as 100% activity. Performance was calculated considering 30% and 70% cut off activities and Trinity as reference.

Results: The intra-assay correlation of Trinity (r = 0.9841, p < 0.001) and PS (r = 0.9833, p < 0.001) did not differ significantly (p = 0.904). Both assays were closely correlated (r = 0.9799, p < 0.001), with a mean difference of 0.1 U/gHb (95% limit of agreement: - 1.32 to 1.57). At 30% cut off PS had a sensitivity of 100% (95% confidence interval (95 CI) 59.0-100.0) and specificity of 100% (95% CI 91.8 to 100.0), at 70% cut-off of 100% (95% CI 79.4-100.0) and 97.1% (95% CI 84.7-99.9) respectively. The G6PD assay from PS is a reliable alternative to the assay from Trinity.

Original languageEnglish
Article number855
Pages (from-to)1-4
Number of pages4
JournalBMC Research Notes
Volume11
Issue number1
DOIs
Publication statusPublished - 4 Dec 2018

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Glucosephosphate Dehydrogenase
Spectrophotometry
Assays
Bangladesh
Ireland
Confidence Intervals
Students
Blood

Cite this

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title = "Spectrophotometry assays to determine G6PD activity from Trinity Biotech and Pointe Scientific G6PD show good correlation",
abstract = "Objectives: Spectrophotometry kits from Pointe Scientific (PS; USA) were compared to kits from Trinity Biotech (Trinity; Ireland) in 50 venous blood samples from purposively selected individuals in Bangladesh. Repeatability and inter-assay variability were assessed by Students t-test, Bland-Altman plot and Pearson correlation coefficient (r). The median glucose-6-phosphate dehydrogenase (G6PD) activity of all G6PD normal participants was calculated per assay and defined as 100{\%} activity. Performance was calculated considering 30{\%} and 70{\%} cut off activities and Trinity as reference. Results: The intra-assay correlation of Trinity (r = 0.9841, p < 0.001) and PS (r = 0.9833, p < 0.001) did not differ significantly (p = 0.904). Both assays were closely correlated (r = 0.9799, p < 0.001), with a mean difference of 0.1 U/gHb (95{\%} limit of agreement: - 1.32 to 1.57). At 30{\%} cut off PS had a sensitivity of 100{\%} (95{\%} confidence interval (95 CI) 59.0-100.0) and specificity of 100{\%} (95{\%} CI 91.8 to 100.0), at 70{\%} cut-off of 100{\%} (95{\%} CI 79.4-100.0) and 97.1{\%} (95{\%} CI 84.7-99.9) respectively. The G6PD assay from PS is a reliable alternative to the assay from Trinity.",
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Spectrophotometry assays to determine G6PD activity from Trinity Biotech and Pointe Scientific G6PD show good correlation. / Alam, Mohammad Shafiul; Kibria, Mohammad Golam; Jahan, Nusrat; Price, Ric N.; Ley, Benedikt.

In: BMC Research Notes, Vol. 11, No. 1, 855, 04.12.2018, p. 1-4.

Research output: Contribution to journalComment/debateResearchpeer-review

TY - JOUR

T1 - Spectrophotometry assays to determine G6PD activity from Trinity Biotech and Pointe Scientific G6PD show good correlation

AU - Alam, Mohammad Shafiul

AU - Kibria, Mohammad Golam

AU - Jahan, Nusrat

AU - Price, Ric N.

AU - Ley, Benedikt

PY - 2018/12/4

Y1 - 2018/12/4

N2 - Objectives: Spectrophotometry kits from Pointe Scientific (PS; USA) were compared to kits from Trinity Biotech (Trinity; Ireland) in 50 venous blood samples from purposively selected individuals in Bangladesh. Repeatability and inter-assay variability were assessed by Students t-test, Bland-Altman plot and Pearson correlation coefficient (r). The median glucose-6-phosphate dehydrogenase (G6PD) activity of all G6PD normal participants was calculated per assay and defined as 100% activity. Performance was calculated considering 30% and 70% cut off activities and Trinity as reference. Results: The intra-assay correlation of Trinity (r = 0.9841, p < 0.001) and PS (r = 0.9833, p < 0.001) did not differ significantly (p = 0.904). Both assays were closely correlated (r = 0.9799, p < 0.001), with a mean difference of 0.1 U/gHb (95% limit of agreement: - 1.32 to 1.57). At 30% cut off PS had a sensitivity of 100% (95% confidence interval (95 CI) 59.0-100.0) and specificity of 100% (95% CI 91.8 to 100.0), at 70% cut-off of 100% (95% CI 79.4-100.0) and 97.1% (95% CI 84.7-99.9) respectively. The G6PD assay from PS is a reliable alternative to the assay from Trinity.

AB - Objectives: Spectrophotometry kits from Pointe Scientific (PS; USA) were compared to kits from Trinity Biotech (Trinity; Ireland) in 50 venous blood samples from purposively selected individuals in Bangladesh. Repeatability and inter-assay variability were assessed by Students t-test, Bland-Altman plot and Pearson correlation coefficient (r). The median glucose-6-phosphate dehydrogenase (G6PD) activity of all G6PD normal participants was calculated per assay and defined as 100% activity. Performance was calculated considering 30% and 70% cut off activities and Trinity as reference. Results: The intra-assay correlation of Trinity (r = 0.9841, p < 0.001) and PS (r = 0.9833, p < 0.001) did not differ significantly (p = 0.904). Both assays were closely correlated (r = 0.9799, p < 0.001), with a mean difference of 0.1 U/gHb (95% limit of agreement: - 1.32 to 1.57). At 30% cut off PS had a sensitivity of 100% (95% confidence interval (95 CI) 59.0-100.0) and specificity of 100% (95% CI 91.8 to 100.0), at 70% cut-off of 100% (95% CI 79.4-100.0) and 97.1% (95% CI 84.7-99.9) respectively. The G6PD assay from PS is a reliable alternative to the assay from Trinity.

KW - G6PD

KW - Malaria

KW - Spectrophotometry

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U2 - 10.1186/s13104-018-3964-7

DO - 10.1186/s13104-018-3964-7

M3 - Comment/debate

VL - 11

SP - 1

EP - 4

JO - BMC Research Notes

JF - BMC Research Notes

SN - 1756-0500

IS - 1

M1 - 855

ER -