Stylosanthes is a host for several phytoplasmas, one of which shows unique 16S-23S intergenic spacer region heterogeneity

Stephanie Jane De La Rue, Anna Padovan, Karen Gibb

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    Stylosanthes sp. exhibiting characteristic symptoms such as little leaf, witches' broom and ̄oral abnormalities were collected from north Queensland and the Northern Territory, Australia. Previous studies have shown that sweet potato little leaf V4 (SPLL-V4), tomato big bud (TBB), stylosanthes little leaf (StLL) and pigeon pea little leaf (PLL) phytoplasmas are associated with this disease. The detection of an additional phytoplasma type, vigna little leaf (ViLL) is reported herein. The range and severity of symptoms expressed by affected plants is highly variable and is not associated with a particular phytoplasma type. Similarly, host plants infected with a complex of two phytoplasmas did not have unique or more severe symptoms. Of the phytoplasmas associated with stylosanthes little leaf disease, StLL is unique because it lacks the tRNAIle gene which is normally situated in the 16S-23S rRNA intergenic spacer region. This phytoplasma was shown to have a second operon containing the expected tRNAIle gene in all StLL samples examined. Sequence analysis suggests that the two 16S rRNA genes amplified by polymerase chain reaction from StLL samples originate from the same phytoplasma. This the first report of a phytopl- asma having ribosomal operons both with and without an intergenic tRNAIle gene.
    Original languageEnglish
    Pages (from-to)613-619
    Number of pages7
    JournalJournal of Phytopathology
    Volume149
    Issue number10
    DOIs
    Publication statusPublished - Oct 2001

    Fingerprint

    Phytoplasma
    Stylosanthes
    Intergenic DNA
    intergenic DNA
    RNA, Transfer, Ile
    leaves
    signs and symptoms (plants)
    operon
    Pigeon pea little leaf phytoplasma
    Operon
    Mouth Abnormalities
    genes
    ribosomal RNA
    witches' broom
    Northern Territory
    Vigna
    Genes
    Ipomoea batatas
    foliar diseases
    Queensland

    Cite this

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    abstract = "Stylosanthes sp. exhibiting characteristic symptoms such as little leaf, witches' broom and ̄oral abnormalities were collected from north Queensland and the Northern Territory, Australia. Previous studies have shown that sweet potato little leaf V4 (SPLL-V4), tomato big bud (TBB), stylosanthes little leaf (StLL) and pigeon pea little leaf (PLL) phytoplasmas are associated with this disease. The detection of an additional phytoplasma type, vigna little leaf (ViLL) is reported herein. The range and severity of symptoms expressed by affected plants is highly variable and is not associated with a particular phytoplasma type. Similarly, host plants infected with a complex of two phytoplasmas did not have unique or more severe symptoms. Of the phytoplasmas associated with stylosanthes little leaf disease, StLL is unique because it lacks the tRNAIle gene which is normally situated in the 16S-23S rRNA intergenic spacer region. This phytoplasma was shown to have a second operon containing the expected tRNAIle gene in all StLL samples examined. Sequence analysis suggests that the two 16S rRNA genes amplified by polymerase chain reaction from StLL samples originate from the same phytoplasma. This the first report of a phytopl- asma having ribosomal operons both with and without an intergenic tRNAIle gene.",
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    author = "{De La Rue}, {Stephanie Jane} and Anna Padovan and Karen Gibb",
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    Stylosanthes is a host for several phytoplasmas, one of which shows unique 16S-23S intergenic spacer region heterogeneity. / De La Rue, Stephanie Jane; Padovan, Anna; Gibb, Karen.

    In: Journal of Phytopathology, Vol. 149, No. 10, 10.2001, p. 613-619.

    Research output: Contribution to journalArticleResearchpeer-review

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