Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010-2012

Jana Lai, Heather Cook, Teem-Wing Yip, Jeanette Berthelsen, Stephen Gourley, Vicki Krause, Helen Smith, Amanda Leach, Heidi Smith-Vaughan

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    Abstract

    Background: An outbreak of serotype 1 invasive pneumococcal disease (IPD) occurred in Central Australia from October 2010 to the latter part of 2012. Surveillance of serotype 1 carriage was conducted to determine epidemiological features of asymptomatic carriage that could potentially be driving the outbreak. 

    Methods: 
    130 patients and accompanying persons presenting at Alice Springs Hospital Emergency Department consented to nasopharyngeal swab (NPS) collection. NPS were processed by standard methods, including culture, pneumococcal lytA quantitative real-time PCR, serotype 1-specific real-time PCR and multi-locus sequence typing (MLST). 

    Results: 
    Pneumococcal carriage was detected in 16% of participants. Carriage was highest in the under 10 year olds from remote communities surrounding Alice Springs (75%). Four NPS were positive for serotype 1 DNA by PCR; 3 were also culture-positive for serotype 1 pneumococci. Serotype 1 isolates had atypical colony morphology on primary culture. All serotype 1 carriers were healthy children 5 to 8 years of age from remote communities. By MLST, serotype 1 isolates were ST306, as were IPD isolates associated with this outbreak. 

    Conclusions: 
    During an outbreak of serotype 1 ST306 IPD, carriage of the outbreak strain was detected in 3% NPS collected. All carriers were healthy children 5 to 8 years of age
    Original languageEnglish
    Article number409
    Pages (from-to)1-7
    Number of pages7
    JournalBMC Infectious Diseases
    Volume13
    DOIs
    Publication statusPublished - 2013

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    Disease Outbreaks
    Real-Time Polymerase Chain Reaction
    Serogroup
    Hospital Departments
    Streptococcus pneumoniae
    Hospital Emergency Service
    Polymerase Chain Reaction
    DNA

    Cite this

    Lai, Jana ; Cook, Heather ; Yip, Teem-Wing ; Berthelsen, Jeanette ; Gourley, Stephen ; Krause, Vicki ; Smith, Helen ; Leach, Amanda ; Smith-Vaughan, Heidi. / Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010-2012. In: BMC Infectious Diseases. 2013 ; Vol. 13. pp. 1-7.
    @article{5e4b8b8ec834433d8ca3e9eeec101d41,
    title = "Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010-2012",
    abstract = "Background: An outbreak of serotype 1 invasive pneumococcal disease (IPD) occurred in Central Australia from October 2010 to the latter part of 2012. Surveillance of serotype 1 carriage was conducted to determine epidemiological features of asymptomatic carriage that could potentially be driving the outbreak. Methods: 130 patients and accompanying persons presenting at Alice Springs Hospital Emergency Department consented to nasopharyngeal swab (NPS) collection. NPS were processed by standard methods, including culture, pneumococcal lytA quantitative real-time PCR, serotype 1-specific real-time PCR and multi-locus sequence typing (MLST). Results: Pneumococcal carriage was detected in 16{\%} of participants. Carriage was highest in the under 10 year olds from remote communities surrounding Alice Springs (75{\%}). Four NPS were positive for serotype 1 DNA by PCR; 3 were also culture-positive for serotype 1 pneumococci. Serotype 1 isolates had atypical colony morphology on primary culture. All serotype 1 carriers were healthy children 5 to 8 years of age from remote communities. By MLST, serotype 1 isolates were ST306, as were IPD isolates associated with this outbreak. Conclusions: During an outbreak of serotype 1 ST306 IPD, carriage of the outbreak strain was detected in 3{\%} NPS collected. All carriers were healthy children 5 to 8 years of age",
    keywords = "DNA, Pneumococcus vaccine, adolescent, adult, aged, article, Australia, bacterium culture, bacterium isolate, child, community, DNA extraction, emergency ward, epidemic, female, human, major clinical study, male, multilocus sequence typing, nasopharyngeal swab, nonhuman, pneumococcal infection, preschool child, real time polymerase chain reaction, school child, serotype, smear, Streptococcus pneumoniae, vaccination, Adolescent, Adult, Aged, Carrier State, Child, Child, Preschool, Disease Outbreaks, Epidemiological Monitoring, Female, Humans, Infant, Male, Middle Aged, Nasopharynx, Pneumococcal Infections, Young Adult",
    author = "Jana Lai and Heather Cook and Teem-Wing Yip and Jeanette Berthelsen and Stephen Gourley and Vicki Krause and Helen Smith and Amanda Leach and Heidi Smith-Vaughan",
    note = "NHMRC Grant No.: 545232, 1024175, 1020561.",
    year = "2013",
    doi = "10.1186/1471-2334-13-409",
    language = "English",
    volume = "13",
    pages = "1--7",
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    Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010-2012. / Lai, Jana; Cook, Heather; Yip, Teem-Wing; Berthelsen, Jeanette; Gourley, Stephen; Krause, Vicki; Smith, Helen; Leach, Amanda; Smith-Vaughan, Heidi.

    In: BMC Infectious Diseases, Vol. 13, 409, 2013, p. 1-7.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010-2012

    AU - Lai, Jana

    AU - Cook, Heather

    AU - Yip, Teem-Wing

    AU - Berthelsen, Jeanette

    AU - Gourley, Stephen

    AU - Krause, Vicki

    AU - Smith, Helen

    AU - Leach, Amanda

    AU - Smith-Vaughan, Heidi

    N1 - NHMRC Grant No.: 545232, 1024175, 1020561.

    PY - 2013

    Y1 - 2013

    N2 - Background: An outbreak of serotype 1 invasive pneumococcal disease (IPD) occurred in Central Australia from October 2010 to the latter part of 2012. Surveillance of serotype 1 carriage was conducted to determine epidemiological features of asymptomatic carriage that could potentially be driving the outbreak. Methods: 130 patients and accompanying persons presenting at Alice Springs Hospital Emergency Department consented to nasopharyngeal swab (NPS) collection. NPS were processed by standard methods, including culture, pneumococcal lytA quantitative real-time PCR, serotype 1-specific real-time PCR and multi-locus sequence typing (MLST). Results: Pneumococcal carriage was detected in 16% of participants. Carriage was highest in the under 10 year olds from remote communities surrounding Alice Springs (75%). Four NPS were positive for serotype 1 DNA by PCR; 3 were also culture-positive for serotype 1 pneumococci. Serotype 1 isolates had atypical colony morphology on primary culture. All serotype 1 carriers were healthy children 5 to 8 years of age from remote communities. By MLST, serotype 1 isolates were ST306, as were IPD isolates associated with this outbreak. Conclusions: During an outbreak of serotype 1 ST306 IPD, carriage of the outbreak strain was detected in 3% NPS collected. All carriers were healthy children 5 to 8 years of age

    AB - Background: An outbreak of serotype 1 invasive pneumococcal disease (IPD) occurred in Central Australia from October 2010 to the latter part of 2012. Surveillance of serotype 1 carriage was conducted to determine epidemiological features of asymptomatic carriage that could potentially be driving the outbreak. Methods: 130 patients and accompanying persons presenting at Alice Springs Hospital Emergency Department consented to nasopharyngeal swab (NPS) collection. NPS were processed by standard methods, including culture, pneumococcal lytA quantitative real-time PCR, serotype 1-specific real-time PCR and multi-locus sequence typing (MLST). Results: Pneumococcal carriage was detected in 16% of participants. Carriage was highest in the under 10 year olds from remote communities surrounding Alice Springs (75%). Four NPS were positive for serotype 1 DNA by PCR; 3 were also culture-positive for serotype 1 pneumococci. Serotype 1 isolates had atypical colony morphology on primary culture. All serotype 1 carriers were healthy children 5 to 8 years of age from remote communities. By MLST, serotype 1 isolates were ST306, as were IPD isolates associated with this outbreak. Conclusions: During an outbreak of serotype 1 ST306 IPD, carriage of the outbreak strain was detected in 3% NPS collected. All carriers were healthy children 5 to 8 years of age

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    KW - adult

    KW - aged

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    KW - major clinical study

    KW - male

    KW - multilocus sequence typing

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    KW - real time polymerase chain reaction

    KW - school child

    KW - serotype

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    KW - Female

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