Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010-2012

Jana Lai, Heather Cook, Teem-Wing Yip, Jeanette Berthelsen, Stephen Gourley, Vicki Krause, Helen Smith, Amanda Leach, Heidi Smith-Vaughan

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Abstract

Background: An outbreak of serotype 1 invasive pneumococcal disease (IPD) occurred in Central Australia from October 2010 to the latter part of 2012. Surveillance of serotype 1 carriage was conducted to determine epidemiological features of asymptomatic carriage that could potentially be driving the outbreak. 

Methods: 
130 patients and accompanying persons presenting at Alice Springs Hospital Emergency Department consented to nasopharyngeal swab (NPS) collection. NPS were processed by standard methods, including culture, pneumococcal lytA quantitative real-time PCR, serotype 1-specific real-time PCR and multi-locus sequence typing (MLST). 

Results: 
Pneumococcal carriage was detected in 16% of participants. Carriage was highest in the under 10 year olds from remote communities surrounding Alice Springs (75%). Four NPS were positive for serotype 1 DNA by PCR; 3 were also culture-positive for serotype 1 pneumococci. Serotype 1 isolates had atypical colony morphology on primary culture. All serotype 1 carriers were healthy children 5 to 8 years of age from remote communities. By MLST, serotype 1 isolates were ST306, as were IPD isolates associated with this outbreak. 

Conclusions: 
During an outbreak of serotype 1 ST306 IPD, carriage of the outbreak strain was detected in 3% NPS collected. All carriers were healthy children 5 to 8 years of age
Original languageEnglish
Article number409
Pages (from-to)1-7
Number of pages7
JournalBMC Infectious Diseases
Volume13
DOIs
Publication statusPublished - 2013

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Disease Outbreaks
Real-Time Polymerase Chain Reaction
Serogroup
Hospital Departments
Streptococcus pneumoniae
Hospital Emergency Service
Polymerase Chain Reaction
DNA

Cite this

Lai, Jana ; Cook, Heather ; Yip, Teem-Wing ; Berthelsen, Jeanette ; Gourley, Stephen ; Krause, Vicki ; Smith, Helen ; Leach, Amanda ; Smith-Vaughan, Heidi. / Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010-2012. In: BMC Infectious Diseases. 2013 ; Vol. 13. pp. 1-7.
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title = "Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010-2012",
abstract = "Background: An outbreak of serotype 1 invasive pneumococcal disease (IPD) occurred in Central Australia from October 2010 to the latter part of 2012. Surveillance of serotype 1 carriage was conducted to determine epidemiological features of asymptomatic carriage that could potentially be driving the outbreak. Methods: 130 patients and accompanying persons presenting at Alice Springs Hospital Emergency Department consented to nasopharyngeal swab (NPS) collection. NPS were processed by standard methods, including culture, pneumococcal lytA quantitative real-time PCR, serotype 1-specific real-time PCR and multi-locus sequence typing (MLST). Results: Pneumococcal carriage was detected in 16{\%} of participants. Carriage was highest in the under 10 year olds from remote communities surrounding Alice Springs (75{\%}). Four NPS were positive for serotype 1 DNA by PCR; 3 were also culture-positive for serotype 1 pneumococci. Serotype 1 isolates had atypical colony morphology on primary culture. All serotype 1 carriers were healthy children 5 to 8 years of age from remote communities. By MLST, serotype 1 isolates were ST306, as were IPD isolates associated with this outbreak. Conclusions: During an outbreak of serotype 1 ST306 IPD, carriage of the outbreak strain was detected in 3{\%} NPS collected. All carriers were healthy children 5 to 8 years of age",
keywords = "DNA, Pneumococcus vaccine, adolescent, adult, aged, article, Australia, bacterium culture, bacterium isolate, child, community, DNA extraction, emergency ward, epidemic, female, human, major clinical study, male, multilocus sequence typing, nasopharyngeal swab, nonhuman, pneumococcal infection, preschool child, real time polymerase chain reaction, school child, serotype, smear, Streptococcus pneumoniae, vaccination, Adolescent, Adult, Aged, Carrier State, Child, Child, Preschool, Disease Outbreaks, Epidemiological Monitoring, Female, Humans, Infant, Male, Middle Aged, Nasopharynx, Pneumococcal Infections, Young Adult",
author = "Jana Lai and Heather Cook and Teem-Wing Yip and Jeanette Berthelsen and Stephen Gourley and Vicki Krause and Helen Smith and Amanda Leach and Heidi Smith-Vaughan",
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Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010-2012. / Lai, Jana; Cook, Heather; Yip, Teem-Wing; Berthelsen, Jeanette; Gourley, Stephen; Krause, Vicki; Smith, Helen; Leach, Amanda; Smith-Vaughan, Heidi.

In: BMC Infectious Diseases, Vol. 13, 409, 2013, p. 1-7.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010-2012

AU - Lai, Jana

AU - Cook, Heather

AU - Yip, Teem-Wing

AU - Berthelsen, Jeanette

AU - Gourley, Stephen

AU - Krause, Vicki

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AU - Leach, Amanda

AU - Smith-Vaughan, Heidi

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N2 - Background: An outbreak of serotype 1 invasive pneumococcal disease (IPD) occurred in Central Australia from October 2010 to the latter part of 2012. Surveillance of serotype 1 carriage was conducted to determine epidemiological features of asymptomatic carriage that could potentially be driving the outbreak. Methods: 130 patients and accompanying persons presenting at Alice Springs Hospital Emergency Department consented to nasopharyngeal swab (NPS) collection. NPS were processed by standard methods, including culture, pneumococcal lytA quantitative real-time PCR, serotype 1-specific real-time PCR and multi-locus sequence typing (MLST). Results: Pneumococcal carriage was detected in 16% of participants. Carriage was highest in the under 10 year olds from remote communities surrounding Alice Springs (75%). Four NPS were positive for serotype 1 DNA by PCR; 3 were also culture-positive for serotype 1 pneumococci. Serotype 1 isolates had atypical colony morphology on primary culture. All serotype 1 carriers were healthy children 5 to 8 years of age from remote communities. By MLST, serotype 1 isolates were ST306, as were IPD isolates associated with this outbreak. Conclusions: During an outbreak of serotype 1 ST306 IPD, carriage of the outbreak strain was detected in 3% NPS collected. All carriers were healthy children 5 to 8 years of age

AB - Background: An outbreak of serotype 1 invasive pneumococcal disease (IPD) occurred in Central Australia from October 2010 to the latter part of 2012. Surveillance of serotype 1 carriage was conducted to determine epidemiological features of asymptomatic carriage that could potentially be driving the outbreak. Methods: 130 patients and accompanying persons presenting at Alice Springs Hospital Emergency Department consented to nasopharyngeal swab (NPS) collection. NPS were processed by standard methods, including culture, pneumococcal lytA quantitative real-time PCR, serotype 1-specific real-time PCR and multi-locus sequence typing (MLST). Results: Pneumococcal carriage was detected in 16% of participants. Carriage was highest in the under 10 year olds from remote communities surrounding Alice Springs (75%). Four NPS were positive for serotype 1 DNA by PCR; 3 were also culture-positive for serotype 1 pneumococci. Serotype 1 isolates had atypical colony morphology on primary culture. All serotype 1 carriers were healthy children 5 to 8 years of age from remote communities. By MLST, serotype 1 isolates were ST306, as were IPD isolates associated with this outbreak. Conclusions: During an outbreak of serotype 1 ST306 IPD, carriage of the outbreak strain was detected in 3% NPS collected. All carriers were healthy children 5 to 8 years of age

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