Synthesis and characterization of gold-conjugated Backhousia citriodora nanoparticles and their anticancer activity against MCF-7 breast and HepG2 liver cancer cell lines

Roshanak Khandanlou, Vinuthaa Murthy, Dhananjaya Saranath, Hetal Damani

    Research output: Contribution to journalArticle

    Abstract

    Environmentally benign-synthesized gold nanoparticles (Au-NPs) have received substantial attention owing to their biomedical applications, particularly in cancer therapy. In the current study, Backhousia citriodora (B. citriodora) leaf extract was applied as a reducing agent for one-pot synthesis of controlled size Au-NPs. The effect of various parameters such as reaction time, pH, and B. citriodora leaf boiling time on the synthesis of Au-NPs was studied. The characterization of the Au-NPs synthesized at 15.0-min incubation time showed colour change because of the surface plasma resonance band around 530.0 nm. TEM photographs showed spherical morphologies with an average size of 8.40 ± 0.084 nm and zeta potential value was − 29.74 mV, indicating stability of the nanoparticles. The biomedical properties of Au-NPs and B. citriodora leaf extract showed strong DPPH radical scavenging. The in vitro anticancer activity determined using MTT assay exhibited that Au-NPs showed a significant dose-dependent reduction in the viability of the MCF-7 breast cancer cell line and the HepG2 liver cancer cell line with IC50 values of 116.65 and 108.21 µg, respectively.

    Original languageEnglish
    Pages (from-to)3106-3118
    Number of pages13
    JournalJournal of Materials Science
    Volume53
    Issue number5
    DOIs
    Publication statusPublished - Mar 2018

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