Systematic Review and Consensus Guidelines for Environmental Sampling of Burkholderia pseudomallei

Direk Limmathurotsakul, David Dance, Vanaporn Wuthiekanun, Mirjam Kaestli, Mark Mayo, Jeffrey Warner, David M Wagner, Apichai Tuanyok, Heiman Wertheim, Tan Yoke Cheng, Chiranjay Mukhopadhyay, S Puthucheary, Nicholas Day, I Steinmetz, Bart Currie, Sharon J Peacock

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    Abstract

    Background: Burkholderia pseudomallei, a Tier 1 Select Agent and the cause of melioidosis, is a Gram-negative bacillus present in the environment in many tropical countries. Defining the global pattern of B. pseudomallei distribution underpins efforts to prevent infection, and is dependent upon robust environmental sampling methodology. Our objective was to review the literature on the detection of environmental B. pseudomallei, update the risk map for melioidosis, and propose international consensus guidelines for soil sampling.

    Methods/Principal Findings: 
    An international working party (Detection of Environmental Burkholderia pseudomallei Working Party (DEBWorP)) was formed during the VIth World Melioidosis Congress in 2010. PubMed (January 1912 to December 2011) was searched using the following MeSH terms: pseudomallei or melioidosis. Bibliographies were hand-searched for secondary references. The reported geographical distribution of B. pseudomallei in the environment was mapped and categorized as definite, probable, or possible. The methodology used for detecting environmental B. pseudomallei was extracted and collated. We found that global coverage was patchy, with a lack of studies in many areas where melioidosis is suspected to occur. The sampling strategies and bacterial identification methods used were highly variable, and not all were robust. We developed consensus guidelines with the goals of reducing the probability of false-negative results, and the provision of affordable and ‘low-tech’ methodology that is applicable in both developed and developing countries.

    Conclusions/Significance: 
    The proposed consensus guidelines provide the basis for the development of an accurate and comprehensive global map of environmental B. pseudomallei.
    Original languageEnglish
    Pages (from-to)e2105-1-e2105-11
    Number of pages11
    JournalPLoS Neglected Tropical Diseases
    Volume7
    Issue number3
    DOIs
    Publication statusPublished - 21 Mar 2013

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    Burkholderia pseudomallei
    Melioidosis
    Guidelines
    Bibliography
    Developed Countries
    PubMed
    Bacillus
    Developing Countries
    Soil
    Infection

    Cite this

    Limmathurotsakul, Direk ; Dance, David ; Wuthiekanun, Vanaporn ; Kaestli, Mirjam ; Mayo, Mark ; Warner, Jeffrey ; Wagner, David M ; Tuanyok, Apichai ; Wertheim, Heiman ; Cheng, Tan Yoke ; Mukhopadhyay, Chiranjay ; Puthucheary, S ; Day, Nicholas ; Steinmetz, I ; Currie, Bart ; Peacock, Sharon J. / Systematic Review and Consensus Guidelines for Environmental Sampling of Burkholderia pseudomallei. In: PLoS Neglected Tropical Diseases. 2013 ; Vol. 7, No. 3. pp. e2105-1-e2105-11.
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    title = "Systematic Review and Consensus Guidelines for Environmental Sampling of Burkholderia pseudomallei",
    abstract = "Background: Burkholderia pseudomallei, a Tier 1 Select Agent and the cause of melioidosis, is a Gram-negative bacillus present in the environment in many tropical countries. Defining the global pattern of B. pseudomallei distribution underpins efforts to prevent infection, and is dependent upon robust environmental sampling methodology. Our objective was to review the literature on the detection of environmental B. pseudomallei, update the risk map for melioidosis, and propose international consensus guidelines for soil sampling.Methods/Principal Findings: An international working party (Detection of Environmental Burkholderia pseudomallei Working Party (DEBWorP)) was formed during the VIth World Melioidosis Congress in 2010. PubMed (January 1912 to December 2011) was searched using the following MeSH terms: pseudomallei or melioidosis. Bibliographies were hand-searched for secondary references. The reported geographical distribution of B. pseudomallei in the environment was mapped and categorized as definite, probable, or possible. The methodology used for detecting environmental B. pseudomallei was extracted and collated. We found that global coverage was patchy, with a lack of studies in many areas where melioidosis is suspected to occur. The sampling strategies and bacterial identification methods used were highly variable, and not all were robust. We developed consensus guidelines with the goals of reducing the probability of false-negative results, and the provision of affordable and ‘low-tech’ methodology that is applicable in both developed and developing countries.Conclusions/Significance: The proposed consensus guidelines provide the basis for the development of an accurate and comprehensive global map of environmental B. pseudomallei.",
    author = "Direk Limmathurotsakul and David Dance and Vanaporn Wuthiekanun and Mirjam Kaestli and Mark Mayo and Jeffrey Warner and Wagner, {David M} and Apichai Tuanyok and Heiman Wertheim and Cheng, {Tan Yoke} and Chiranjay Mukhopadhyay and S Puthucheary and Nicholas Day and I Steinmetz and Bart Currie and Peacock, {Sharon J}",
    year = "2013",
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    Limmathurotsakul, D, Dance, D, Wuthiekanun, V, Kaestli, M, Mayo, M, Warner, J, Wagner, DM, Tuanyok, A, Wertheim, H, Cheng, TY, Mukhopadhyay, C, Puthucheary, S, Day, N, Steinmetz, I, Currie, B & Peacock, SJ 2013, 'Systematic Review and Consensus Guidelines for Environmental Sampling of Burkholderia pseudomallei', PLoS Neglected Tropical Diseases, vol. 7, no. 3, pp. e2105-1-e2105-11. https://doi.org/10.1371/journal.pntd.0002105

    Systematic Review and Consensus Guidelines for Environmental Sampling of Burkholderia pseudomallei. / Limmathurotsakul, Direk; Dance, David; Wuthiekanun, Vanaporn; Kaestli, Mirjam; Mayo, Mark; Warner, Jeffrey; Wagner, David M; Tuanyok, Apichai; Wertheim, Heiman; Cheng, Tan Yoke; Mukhopadhyay, Chiranjay; Puthucheary, S; Day, Nicholas; Steinmetz, I; Currie, Bart; Peacock, Sharon J.

    In: PLoS Neglected Tropical Diseases, Vol. 7, No. 3, 21.03.2013, p. e2105-1-e2105-11.

    Research output: Contribution to journalArticleResearchpeer-review

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    AU - Limmathurotsakul, Direk

    AU - Dance, David

    AU - Wuthiekanun, Vanaporn

    AU - Kaestli, Mirjam

    AU - Mayo, Mark

    AU - Warner, Jeffrey

    AU - Wagner, David M

    AU - Tuanyok, Apichai

    AU - Wertheim, Heiman

    AU - Cheng, Tan Yoke

    AU - Mukhopadhyay, Chiranjay

    AU - Puthucheary, S

    AU - Day, Nicholas

    AU - Steinmetz, I

    AU - Currie, Bart

    AU - Peacock, Sharon J

    PY - 2013/3/21

    Y1 - 2013/3/21

    N2 - Background: Burkholderia pseudomallei, a Tier 1 Select Agent and the cause of melioidosis, is a Gram-negative bacillus present in the environment in many tropical countries. Defining the global pattern of B. pseudomallei distribution underpins efforts to prevent infection, and is dependent upon robust environmental sampling methodology. Our objective was to review the literature on the detection of environmental B. pseudomallei, update the risk map for melioidosis, and propose international consensus guidelines for soil sampling.Methods/Principal Findings: An international working party (Detection of Environmental Burkholderia pseudomallei Working Party (DEBWorP)) was formed during the VIth World Melioidosis Congress in 2010. PubMed (January 1912 to December 2011) was searched using the following MeSH terms: pseudomallei or melioidosis. Bibliographies were hand-searched for secondary references. The reported geographical distribution of B. pseudomallei in the environment was mapped and categorized as definite, probable, or possible. The methodology used for detecting environmental B. pseudomallei was extracted and collated. We found that global coverage was patchy, with a lack of studies in many areas where melioidosis is suspected to occur. The sampling strategies and bacterial identification methods used were highly variable, and not all were robust. We developed consensus guidelines with the goals of reducing the probability of false-negative results, and the provision of affordable and ‘low-tech’ methodology that is applicable in both developed and developing countries.Conclusions/Significance: The proposed consensus guidelines provide the basis for the development of an accurate and comprehensive global map of environmental B. pseudomallei.

    AB - Background: Burkholderia pseudomallei, a Tier 1 Select Agent and the cause of melioidosis, is a Gram-negative bacillus present in the environment in many tropical countries. Defining the global pattern of B. pseudomallei distribution underpins efforts to prevent infection, and is dependent upon robust environmental sampling methodology. Our objective was to review the literature on the detection of environmental B. pseudomallei, update the risk map for melioidosis, and propose international consensus guidelines for soil sampling.Methods/Principal Findings: An international working party (Detection of Environmental Burkholderia pseudomallei Working Party (DEBWorP)) was formed during the VIth World Melioidosis Congress in 2010. PubMed (January 1912 to December 2011) was searched using the following MeSH terms: pseudomallei or melioidosis. Bibliographies were hand-searched for secondary references. The reported geographical distribution of B. pseudomallei in the environment was mapped and categorized as definite, probable, or possible. The methodology used for detecting environmental B. pseudomallei was extracted and collated. We found that global coverage was patchy, with a lack of studies in many areas where melioidosis is suspected to occur. The sampling strategies and bacterial identification methods used were highly variable, and not all were robust. We developed consensus guidelines with the goals of reducing the probability of false-negative results, and the provision of affordable and ‘low-tech’ methodology that is applicable in both developed and developing countries.Conclusions/Significance: The proposed consensus guidelines provide the basis for the development of an accurate and comprehensive global map of environmental B. pseudomallei.

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