The chitinase allergens Der p 15 and Der p 18 from Dermatophagoides pteronyssinus

Susan O'Neill, T Heinrich, Belinda Hales, L Hazell, Deborah Holt, K FISCHER, Wayne Thomas

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background: House dust mites Dermatophagoides pteronyssinus and Dermatophagoides farinae cause allergic disease in humans as well as in dogs. In geographical regions where the two mite species coexist, they both elicit specific immunoglobulin (Ig E) responses in humans whereas dogs preferentially react to D. farinae extracts. In dogs the main IgE binding is directed to the D. farinae chitinase allergens Der f 15 and Der f 18 and not to the groups 1 and 2 allergens as found for humans. Although the IgE response of humans to Der f 18 has been investigated there is no report on Der f 15-specific IgE in humans. Objective: This study aimed to characterize the chitinase allergens Der p 15 and Der p 18 of D. pteronyssinus and to find out whether they are important allergens for humans. Methods: cDNA was cloned by a polymerase chain reaction strategy from D. pteronyssinus libraries using primers based on conserved chitinase sequences. IgE binding to the recombinant polypeptides was measured by immunosorbent assay. Mice were immunized with the polypeptides and cross-reactivity examined. Results: Two variants of Der p 15 were isolated, encoding mature proteins of 58.8 and 61.4 kDa. The amino acid sequences had 90% identity to Der f 15. The cDNA for Der p 18 encoded a mature protein of 49.2 kDa with 88% sequence identity to Der f 18. Der p 15-specific IgE was detected in 70% and Der p 18-specific IgE in 63% of a panel of 27 human allergic sera. Conclusions: The D. pteronyssinus chitinases Der p 15 and Der p 18 show a high frequency of binding to IgE in allergic human sera. They are therefore potentially important allergens for humans as well as dogs. � 2006 The Authors.
Original languageEnglish
Pages (from-to)831-839
Number of pages9
JournalClinical and Experimental Allergy
Volume36
Issue number6
Publication statusPublished - 2006

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Dermatophagoides pteronyssinus
Chitinases
Allergens
Immunoglobulin E
Dermatophagoides farinae
Dogs
Complementary DNA
Dermatophagoides Antigens
Pyroglyphidae
Immunosorbents
Peptides
Conserved Sequence
Mites
Serum
Libraries
Amino Acid Sequence
Proteins
Polymerase Chain Reaction

Cite this

O'Neill, S., Heinrich, T., Hales, B., Hazell, L., Holt, D., FISCHER, K., & Thomas, W. (2006). The chitinase allergens Der p 15 and Der p 18 from Dermatophagoides pteronyssinus. Clinical and Experimental Allergy, 36(6), 831-839.
O'Neill, Susan ; Heinrich, T ; Hales, Belinda ; Hazell, L ; Holt, Deborah ; FISCHER, K ; Thomas, Wayne. / The chitinase allergens Der p 15 and Der p 18 from Dermatophagoides pteronyssinus. In: Clinical and Experimental Allergy. 2006 ; Vol. 36, No. 6. pp. 831-839.
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O'Neill, S, Heinrich, T, Hales, B, Hazell, L, Holt, D, FISCHER, K & Thomas, W 2006, 'The chitinase allergens Der p 15 and Der p 18 from Dermatophagoides pteronyssinus', Clinical and Experimental Allergy, vol. 36, no. 6, pp. 831-839.

The chitinase allergens Der p 15 and Der p 18 from Dermatophagoides pteronyssinus. / O'Neill, Susan; Heinrich, T; Hales, Belinda; Hazell, L; Holt, Deborah; FISCHER, K; Thomas, Wayne.

In: Clinical and Experimental Allergy, Vol. 36, No. 6, 2006, p. 831-839.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - The chitinase allergens Der p 15 and Der p 18 from Dermatophagoides pteronyssinus

AU - O'Neill, Susan

AU - Heinrich, T

AU - Hales, Belinda

AU - Hazell, L

AU - Holt, Deborah

AU - FISCHER, K

AU - Thomas, Wayne

PY - 2006

Y1 - 2006

N2 - Background: House dust mites Dermatophagoides pteronyssinus and Dermatophagoides farinae cause allergic disease in humans as well as in dogs. In geographical regions where the two mite species coexist, they both elicit specific immunoglobulin (Ig E) responses in humans whereas dogs preferentially react to D. farinae extracts. In dogs the main IgE binding is directed to the D. farinae chitinase allergens Der f 15 and Der f 18 and not to the groups 1 and 2 allergens as found for humans. Although the IgE response of humans to Der f 18 has been investigated there is no report on Der f 15-specific IgE in humans. Objective: This study aimed to characterize the chitinase allergens Der p 15 and Der p 18 of D. pteronyssinus and to find out whether they are important allergens for humans. Methods: cDNA was cloned by a polymerase chain reaction strategy from D. pteronyssinus libraries using primers based on conserved chitinase sequences. IgE binding to the recombinant polypeptides was measured by immunosorbent assay. Mice were immunized with the polypeptides and cross-reactivity examined. Results: Two variants of Der p 15 were isolated, encoding mature proteins of 58.8 and 61.4 kDa. The amino acid sequences had 90% identity to Der f 15. The cDNA for Der p 18 encoded a mature protein of 49.2 kDa with 88% sequence identity to Der f 18. Der p 15-specific IgE was detected in 70% and Der p 18-specific IgE in 63% of a panel of 27 human allergic sera. Conclusions: The D. pteronyssinus chitinases Der p 15 and Der p 18 show a high frequency of binding to IgE in allergic human sera. They are therefore potentially important allergens for humans as well as dogs. � 2006 The Authors.

AB - Background: House dust mites Dermatophagoides pteronyssinus and Dermatophagoides farinae cause allergic disease in humans as well as in dogs. In geographical regions where the two mite species coexist, they both elicit specific immunoglobulin (Ig E) responses in humans whereas dogs preferentially react to D. farinae extracts. In dogs the main IgE binding is directed to the D. farinae chitinase allergens Der f 15 and Der f 18 and not to the groups 1 and 2 allergens as found for humans. Although the IgE response of humans to Der f 18 has been investigated there is no report on Der f 15-specific IgE in humans. Objective: This study aimed to characterize the chitinase allergens Der p 15 and Der p 18 of D. pteronyssinus and to find out whether they are important allergens for humans. Methods: cDNA was cloned by a polymerase chain reaction strategy from D. pteronyssinus libraries using primers based on conserved chitinase sequences. IgE binding to the recombinant polypeptides was measured by immunosorbent assay. Mice were immunized with the polypeptides and cross-reactivity examined. Results: Two variants of Der p 15 were isolated, encoding mature proteins of 58.8 and 61.4 kDa. The amino acid sequences had 90% identity to Der f 15. The cDNA for Der p 18 encoded a mature protein of 49.2 kDa with 88% sequence identity to Der f 18. Der p 15-specific IgE was detected in 70% and Der p 18-specific IgE in 63% of a panel of 27 human allergic sera. Conclusions: The D. pteronyssinus chitinases Der p 15 and Der p 18 show a high frequency of binding to IgE in allergic human sera. They are therefore potentially important allergens for humans as well as dogs. � 2006 The Authors.

KW - chitinase

KW - complementary DNA

KW - Der p 15 allergen

KW - Der p 18 allergen

KW - house dust allergen

KW - immunoglobulin E

KW - polypeptide

KW - recombinant protein

KW - unclassified drug

KW - allergy

KW - amino acid sequence

KW - article

KW - clinical article

KW - controlled study

KW - cross reaction

KW - Dermatophagoides farinae

KW - Dermatophagoides pteronyssinus

KW - dog

KW - enzyme linked immunosorbent assay

KW - human

KW - immune response

KW - molecular cloning

KW - nonhuman

KW - nucleotide sequence

KW - polymerase chain reaction

KW - priority journal

KW - protein binding

KW - protein isolation

KW - sequence homology

KW - Amino Acid Sequence

KW - Animals

KW - Antigens, Dermatophagoides

KW - Base Sequence

KW - Case-Control Studies

KW - Chitinase

KW - Cloning, Molecular

KW - Cross Reactions

KW - DNA, Complementary

KW - Gene Library

KW - Humans

KW - Hypersensitivity

KW - Immunoglobulin E

KW - Mice

KW - Mice, Inbred C57BL

KW - Molecular Sequence Data

KW - Recombinant Proteins

KW - Sequence Alignment

KW - Sequence Analysis, DNA

M3 - Article

VL - 36

SP - 831

EP - 839

JO - Clinical and Experimental Allergy

JF - Clinical and Experimental Allergy

SN - 0954-7894

IS - 6

ER -

O'Neill S, Heinrich T, Hales B, Hazell L, Holt D, FISCHER K et al. The chitinase allergens Der p 15 and Der p 18 from Dermatophagoides pteronyssinus. Clinical and Experimental Allergy. 2006;36(6):831-839.