The complete mitochondrial genome of the mantid shrimp Harpiosquilla harpax, and a phylogenetic investigation of the Decapoda using mitochondrial sequences

A Miller, Chris Austin

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    The complete mitochondrial DNA sequence was determined for the mantid shrimp Harpiosquilla harpax. These data demonstrate that the H. harpax mitochondrial genome is a 15,714 bp circular molecule and encodes the typical 37 metazoan mitochondrial genes (13 protein-coding, 22 tRNA, and two rRNA genes). The gene arrangement of H. harpax is consistent with that of the putative arthropod ancestral gene order as depicted by Limulus polyphemus. H. harpax was employed as an outgroup taxon for a phylogenetic investigation of the Decapoda using sequences from complete mitochondrial genomes. Whilst our results are largely in agreement with current taxonomic treatments, the relationships indicated among the reptantian decapods are novel. Our results provide strong statistical support for a sister-group relationship between the Achelata and the Astacida. These findings not only refute previous phylogenetic hypotheses, but also have serious implications for the interpretation of morphological and developmental evolution in the Decapoda. In addition we also investigated the effects of outgroup selection on the resolution of ingroup relationships. We found outgroup choice to significantly influence tree topology thus reinforcing the importance of appropriate outgroup selection in phylogenetic studies. � 2005 Elsevier Inc. All rights reserved.
    Original languageEnglish
    Pages (from-to)565-574
    Number of pages10
    JournalMolecular Phylogenetics and Evolution
    Volume38
    Issue number3
    Publication statusPublished - 2006

    Fingerprint

    Mantodea
    Gene Order
    Mitochondrial Genome
    Decapoda
    shrimp
    genome
    Decapoda (Crustacea)
    phylogenetics
    Horseshoe Crabs
    Mitochondrial Genes
    Arthropods
    gene
    phylogeny
    Transfer RNA
    Mitochondrial DNA
    rRNA Genes
    genes
    Limulus polyphemus
    metazoan
    mitochondrial DNA

    Cite this

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    title = "The complete mitochondrial genome of the mantid shrimp Harpiosquilla harpax, and a phylogenetic investigation of the Decapoda using mitochondrial sequences",
    abstract = "The complete mitochondrial DNA sequence was determined for the mantid shrimp Harpiosquilla harpax. These data demonstrate that the H. harpax mitochondrial genome is a 15,714 bp circular molecule and encodes the typical 37 metazoan mitochondrial genes (13 protein-coding, 22 tRNA, and two rRNA genes). The gene arrangement of H. harpax is consistent with that of the putative arthropod ancestral gene order as depicted by Limulus polyphemus. H. harpax was employed as an outgroup taxon for a phylogenetic investigation of the Decapoda using sequences from complete mitochondrial genomes. Whilst our results are largely in agreement with current taxonomic treatments, the relationships indicated among the reptantian decapods are novel. Our results provide strong statistical support for a sister-group relationship between the Achelata and the Astacida. These findings not only refute previous phylogenetic hypotheses, but also have serious implications for the interpretation of morphological and developmental evolution in the Decapoda. In addition we also investigated the effects of outgroup selection on the resolution of ingroup relationships. We found outgroup choice to significantly influence tree topology thus reinforcing the importance of appropriate outgroup selection in phylogenetic studies. � 2005 Elsevier Inc. All rights reserved.",
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    author = "A Miller and Chris Austin",
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    The complete mitochondrial genome of the mantid shrimp Harpiosquilla harpax, and a phylogenetic investigation of the Decapoda using mitochondrial sequences. / Miller, A; Austin, Chris.

    In: Molecular Phylogenetics and Evolution, Vol. 38, No. 3, 2006, p. 565-574.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - The complete mitochondrial genome of the mantid shrimp Harpiosquilla harpax, and a phylogenetic investigation of the Decapoda using mitochondrial sequences

    AU - Miller, A

    AU - Austin, Chris

    PY - 2006

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    N2 - The complete mitochondrial DNA sequence was determined for the mantid shrimp Harpiosquilla harpax. These data demonstrate that the H. harpax mitochondrial genome is a 15,714 bp circular molecule and encodes the typical 37 metazoan mitochondrial genes (13 protein-coding, 22 tRNA, and two rRNA genes). The gene arrangement of H. harpax is consistent with that of the putative arthropod ancestral gene order as depicted by Limulus polyphemus. H. harpax was employed as an outgroup taxon for a phylogenetic investigation of the Decapoda using sequences from complete mitochondrial genomes. Whilst our results are largely in agreement with current taxonomic treatments, the relationships indicated among the reptantian decapods are novel. Our results provide strong statistical support for a sister-group relationship between the Achelata and the Astacida. These findings not only refute previous phylogenetic hypotheses, but also have serious implications for the interpretation of morphological and developmental evolution in the Decapoda. In addition we also investigated the effects of outgroup selection on the resolution of ingroup relationships. We found outgroup choice to significantly influence tree topology thus reinforcing the importance of appropriate outgroup selection in phylogenetic studies. � 2005 Elsevier Inc. All rights reserved.

    AB - The complete mitochondrial DNA sequence was determined for the mantid shrimp Harpiosquilla harpax. These data demonstrate that the H. harpax mitochondrial genome is a 15,714 bp circular molecule and encodes the typical 37 metazoan mitochondrial genes (13 protein-coding, 22 tRNA, and two rRNA genes). The gene arrangement of H. harpax is consistent with that of the putative arthropod ancestral gene order as depicted by Limulus polyphemus. H. harpax was employed as an outgroup taxon for a phylogenetic investigation of the Decapoda using sequences from complete mitochondrial genomes. Whilst our results are largely in agreement with current taxonomic treatments, the relationships indicated among the reptantian decapods are novel. Our results provide strong statistical support for a sister-group relationship between the Achelata and the Astacida. These findings not only refute previous phylogenetic hypotheses, but also have serious implications for the interpretation of morphological and developmental evolution in the Decapoda. In addition we also investigated the effects of outgroup selection on the resolution of ingroup relationships. We found outgroup choice to significantly influence tree topology thus reinforcing the importance of appropriate outgroup selection in phylogenetic studies. � 2005 Elsevier Inc. All rights reserved.

    KW - mitochondrial DNA

    KW - primer DNA

    KW - ribosome RNA

    KW - transfer RNA

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    KW - article

    KW - chemistry

    KW - classification

    KW - conformation

    KW - Crustacea

    KW - genetics

    KW - genome

    KW - molecular cloning

    KW - nucleotide sequence

    KW - phylogeny

    KW - polymerase chain reaction

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    KW - Cloning, Molecular

    KW - DNA Primers

    KW - DNA, Mitochondrial

    KW - Genome

    KW - Nucleic Acid Conformation

    KW - Phylogeny

    KW - Polymerase Chain Reaction

    KW - RNA, Ribosomal

    KW - RNA, Transfer

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    KW - Limulus polyphemus

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    KW - Stomatopoda

    M3 - Article

    VL - 38

    SP - 565

    EP - 574

    JO - Molecular Phylogenetics and Evolution

    JF - Molecular Phylogenetics and Evolution

    SN - 1055-7903

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    ER -