The in vitro vascular effects of two chirodropid (Chironex fleckeri and Chiropsella bronzie) venoms

K WINTER, R Fernando, S Ramasamy, J SEYMOUR, Geoffrey Isbister, W HODGSON

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Clinical observations suggest a primary cardiotoxic role in fatal Chironex fleckeri stings. The limited research available indicates that Chiropsella bronzie venom acts in a similar manner although appears to be less potent. The aim of the present study was to elucidate the vascular effects of C. fleckeri and C. bronzie venoms using rat isolated aorta. Both venoms produced a sustained contraction of endothelium-denuded aorta which was not significantly affected by prazosin or box jellyfish antivenom. Felodipine significantly reduced the contractile response to C. fleckeri venom but not C. bronzie venom. Both venoms produced an initial relaxation (Phase 1), followed by a sustained contraction (Phase 2), in pre-contracted endothelium-intact aorta. Removal of the endothelium significantly inhibited both phases of the response. NOLA significantly inhibited Phase 1, but not Phase 2, of the response to both venoms. Atropine, HOE 140 or BQ 123 did not have any significant inhibitory effect on either phase. In conclusion, neither C. fleckeri nor C. bronzie venoms appear to contain components with activity at ?1-adrenoceptors. Antivenom was ineffective in reversing the effects of the venom suggesting it is incapable of completely neutralising nematocyst-derived venom. Determining the mechanism of action of these venoms will allow for the development of better treatment strategies. � 2006 Elsevier Ireland Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)13-20
Number of pages8
JournalToxicology Letters
Volume168
Issue number1
Publication statusPublished - 2007

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Cubozoa
Venoms
Blood Vessels
Endothelium
Antivenins
Aorta
Cnidarian Venoms
In Vitro Techniques
Felodipine
Prazosin
Bites and Stings
Atropine
Ireland
Adrenergic Receptors

Cite this

WINTER, K., Fernando, R., Ramasamy, S., SEYMOUR, J., Isbister, G., & HODGSON, W. (2007). The in vitro vascular effects of two chirodropid (Chironex fleckeri and Chiropsella bronzie) venoms. Toxicology Letters, 168(1), 13-20.
WINTER, K ; Fernando, R ; Ramasamy, S ; SEYMOUR, J ; Isbister, Geoffrey ; HODGSON, W. / The in vitro vascular effects of two chirodropid (Chironex fleckeri and Chiropsella bronzie) venoms. In: Toxicology Letters. 2007 ; Vol. 168, No. 1. pp. 13-20.
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WINTER, K, Fernando, R, Ramasamy, S, SEYMOUR, J, Isbister, G & HODGSON, W 2007, 'The in vitro vascular effects of two chirodropid (Chironex fleckeri and Chiropsella bronzie) venoms', Toxicology Letters, vol. 168, no. 1, pp. 13-20.

The in vitro vascular effects of two chirodropid (Chironex fleckeri and Chiropsella bronzie) venoms. / WINTER, K; Fernando, R; Ramasamy, S; SEYMOUR, J; Isbister, Geoffrey; HODGSON, W.

In: Toxicology Letters, Vol. 168, No. 1, 2007, p. 13-20.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - The in vitro vascular effects of two chirodropid (Chironex fleckeri and Chiropsella bronzie) venoms

AU - WINTER, K

AU - Fernando, R

AU - Ramasamy, S

AU - SEYMOUR, J

AU - Isbister, Geoffrey

AU - HODGSON, W

PY - 2007

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AB - Clinical observations suggest a primary cardiotoxic role in fatal Chironex fleckeri stings. The limited research available indicates that Chiropsella bronzie venom acts in a similar manner although appears to be less potent. The aim of the present study was to elucidate the vascular effects of C. fleckeri and C. bronzie venoms using rat isolated aorta. Both venoms produced a sustained contraction of endothelium-denuded aorta which was not significantly affected by prazosin or box jellyfish antivenom. Felodipine significantly reduced the contractile response to C. fleckeri venom but not C. bronzie venom. Both venoms produced an initial relaxation (Phase 1), followed by a sustained contraction (Phase 2), in pre-contracted endothelium-intact aorta. Removal of the endothelium significantly inhibited both phases of the response. NOLA significantly inhibited Phase 1, but not Phase 2, of the response to both venoms. Atropine, HOE 140 or BQ 123 did not have any significant inhibitory effect on either phase. In conclusion, neither C. fleckeri nor C. bronzie venoms appear to contain components with activity at ?1-adrenoceptors. Antivenom was ineffective in reversing the effects of the venom suggesting it is incapable of completely neutralising nematocyst-derived venom. Determining the mechanism of action of these venoms will allow for the development of better treatment strategies. � 2006 Elsevier Ireland Ltd. All rights reserved.

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KW - cyclo(dextro tryptophyl dextro aspartylprolyl dextro valylleucyl)

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KW - jellyfish

KW - male

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KW - Antivenins

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KW - Cubozoa

KW - Endothelium, Vascular

KW - Enzyme Inhibitors

KW - Felodipine

KW - Male

KW - Nitroarginine

KW - Prazosin

KW - Rats

KW - Rats, Sprague-Dawley

KW - Vasoconstriction

KW - Chirodropidae

KW - Chironex fleckeri

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WINTER K, Fernando R, Ramasamy S, SEYMOUR J, Isbister G, HODGSON W. The in vitro vascular effects of two chirodropid (Chironex fleckeri and Chiropsella bronzie) venoms. Toxicology Letters. 2007;168(1):13-20.