The role of Staphylococcal carotenogenesis in resistance to host defense peptides and in vivo virulence in experimental endocarditis model

Yan Q Xiong, Soo-Jin Yang, Steven Tong, Danya N Alvarez, Nagendra N Mishra

Research output: Contribution to journalComment/debateResearch

Abstract

The defining hallmark of the newly described species, Staphylococcus argenteus, in comparison to its sister species, S. aureus and S. schweitzeri, is the absence of production of the carotenoid pigment, staphyloxanthin. Staphylococcus argenteus lacks the responsible genetic locus crtOPQMN. We examined the impact of carotenoid synthesis in two non-pigmented S. argenteus strains, MSHR1132 and SCC1165. Following complementation with a plasmid containing the carotenoid operon (pTX-crtOPQMN), compared to wild type, both complemented strains showed substantial carotenoid production, with a resultant increase in cell membrane rigidity. Surprisingly, both crtOPQMN-complemented strains exhibited increased susceptibility to the host defense peptides, LL-37 and hNP-1 in vitro, and reduced virulence in an experimental rabbit endocarditis model.
Original languageEnglish
Article numberftv056
Pages (from-to)1-5
Number of pages5
JournalPathogens and Disease
Volume73
Issue number7
DOIs
Publication statusPublished - 4 Aug 2015

Fingerprint

Carotenoids
Endocarditis
Virulence
Theoretical Models
Peptides
Staphylococcus
Genetic Loci
Operon
Plasmids
Cell Membrane
Rabbits

Cite this

@article{32b061dc6090489d9264039748f85c8b,
title = "The role of Staphylococcal carotenogenesis in resistance to host defense peptides and in vivo virulence in experimental endocarditis model",
abstract = "The defining hallmark of the newly described species, Staphylococcus argenteus, in comparison to its sister species, S. aureus and S. schweitzeri, is the absence of production of the carotenoid pigment, staphyloxanthin. Staphylococcus argenteus lacks the responsible genetic locus crtOPQMN. We examined the impact of carotenoid synthesis in two non-pigmented S. argenteus strains, MSHR1132 and SCC1165. Following complementation with a plasmid containing the carotenoid operon (pTX-crtOPQMN), compared to wild type, both complemented strains showed substantial carotenoid production, with a resultant increase in cell membrane rigidity. Surprisingly, both crtOPQMN-complemented strains exhibited increased susceptibility to the host defense peptides, LL-37 and hNP-1 in vitro, and reduced virulence in an experimental rabbit endocarditis model.",
author = "Xiong, {Yan Q} and Soo-Jin Yang and Steven Tong and Alvarez, {Danya N} and Mishra, {Nagendra N}",
year = "2015",
month = "8",
day = "4",
doi = "10.1093/femspd/ftv056",
language = "English",
volume = "73",
pages = "1--5",
journal = "Pathogens and Disease",
issn = "0928-8244",
publisher = "Wiley-Blackwell",
number = "7",

}

The role of Staphylococcal carotenogenesis in resistance to host defense peptides and in vivo virulence in experimental endocarditis model. / Xiong, Yan Q; Yang, Soo-Jin; Tong, Steven; Alvarez, Danya N; Mishra, Nagendra N.

In: Pathogens and Disease, Vol. 73, No. 7, ftv056, 04.08.2015, p. 1-5.

Research output: Contribution to journalComment/debateResearch

TY - JOUR

T1 - The role of Staphylococcal carotenogenesis in resistance to host defense peptides and in vivo virulence in experimental endocarditis model

AU - Xiong, Yan Q

AU - Yang, Soo-Jin

AU - Tong, Steven

AU - Alvarez, Danya N

AU - Mishra, Nagendra N

PY - 2015/8/4

Y1 - 2015/8/4

N2 - The defining hallmark of the newly described species, Staphylococcus argenteus, in comparison to its sister species, S. aureus and S. schweitzeri, is the absence of production of the carotenoid pigment, staphyloxanthin. Staphylococcus argenteus lacks the responsible genetic locus crtOPQMN. We examined the impact of carotenoid synthesis in two non-pigmented S. argenteus strains, MSHR1132 and SCC1165. Following complementation with a plasmid containing the carotenoid operon (pTX-crtOPQMN), compared to wild type, both complemented strains showed substantial carotenoid production, with a resultant increase in cell membrane rigidity. Surprisingly, both crtOPQMN-complemented strains exhibited increased susceptibility to the host defense peptides, LL-37 and hNP-1 in vitro, and reduced virulence in an experimental rabbit endocarditis model.

AB - The defining hallmark of the newly described species, Staphylococcus argenteus, in comparison to its sister species, S. aureus and S. schweitzeri, is the absence of production of the carotenoid pigment, staphyloxanthin. Staphylococcus argenteus lacks the responsible genetic locus crtOPQMN. We examined the impact of carotenoid synthesis in two non-pigmented S. argenteus strains, MSHR1132 and SCC1165. Following complementation with a plasmid containing the carotenoid operon (pTX-crtOPQMN), compared to wild type, both complemented strains showed substantial carotenoid production, with a resultant increase in cell membrane rigidity. Surprisingly, both crtOPQMN-complemented strains exhibited increased susceptibility to the host defense peptides, LL-37 and hNP-1 in vitro, and reduced virulence in an experimental rabbit endocarditis model.

UR - http://www.scopus.com/inward/record.url?scp=85010053739&partnerID=8YFLogxK

U2 - 10.1093/femspd/ftv056

DO - 10.1093/femspd/ftv056

M3 - Comment/debate

VL - 73

SP - 1

EP - 5

JO - Pathogens and Disease

JF - Pathogens and Disease

SN - 0928-8244

IS - 7

M1 - ftv056

ER -