Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens

Leisha J. Richardson, Mirjam Kaestli, Mark Mayo, Jolene R. Bowers, Apichai Tuanyok, Jim Schupp, David Engelthaler, David M. Wagner, Paul S. Keim, Bart J. Currie

    Research output: Contribution to journalArticle

    Abstract

    Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.

    Original languageEnglish
    Pages (from-to)179-181
    Number of pages3
    JournalJournal of Microbiological Methods
    Volume88
    Issue number1
    DOIs
    Publication statusPublished - Jan 2012

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