@article{f395985a2eb946deb43262828b9bc108,
title = "Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens",
abstract = "Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.",
keywords = "bacterial DNA, article, blood analysis, blood buffy coat, Burkholderia pseudomallei, clinical article, controlled study, DNA extraction, human, intermethod comparison, melioidosis, priority journal, process optimization, real time polymerase chain reaction, sputum analysis, type III secretion system, urinalysis, DNA, Bacterial, Humans, Melioidosis, Pathology, Molecular, Real-Time Polymerase Chain Reaction",
author = "Richardson, {Leisha J.} and Mirjam Kaestli and Mark Mayo and Bowers, {Jolene R.} and Apichai Tuanyok and Jim Schupp and David Engelthaler and Wagner, {David M.} and Keim, {Paul S.} and Currie, {Bart J.}",
year = "2012",
month = jan,
doi = "10.1016/j.mimet.2011.10.023",
language = "English",
volume = "88",
pages = "179--181",
journal = "Journal of Microbiological Methods",
issn = "0167-7012",
publisher = "Elsevier",
number = "1",
}