TY - JOUR
T1 - Ultrasmall superparamagnetic Fe3O4 nanoparticles
T2 - honey-based green and facile synthesis and in vitro viability assay
AU - Rasouli, Elisa
AU - Basirun, Wan Jeffrey
AU - Rezayi, Majid
AU - Shameli, Kamyar
AU - Nourmohammadi, Esmail
AU - Khandanlou, Roshanak
AU - Izadiyan, Zahra
AU - Khoshdel Sarkarizi, Hoda
PY - 2018/10/26
Y1 - 2018/10/26
N2 - Introduction: In the present research, we report a quick and green synthesis of magnetite nanoparticles (Fe3O4-NPs) in aqueous solution using ferric and ferrous chloride, with different percentages of natural honey (0.5%, 1.0%, 3.0% and 5.0% w/v) as the precursors, stabilizer, reducing and capping agent, respectively. The effect of the stabilizer on the magnetic properties and size of Fe3O4-NPs was also studied. Methods: The nanoparticles were characterized by X-ray diffraction (XRD) analysis, field emission scanning electron microscopy, energy dispersive X-ray fluorescence, transmission electron microscopy (TEM), vibrating sample magnetometry (VSM) and Fourier transform infrared spectroscopy. Results: The XRD analysis indicated the presence of pure Fe3O4-NPs while the TEM images indicated that the Fe3O4-NPs are spherical with a diameter range between 3.21 and 2.22 nm. The VSM study demonstrated that the magnetic properties were enhanced with the decrease in the percentage of honey. In vitro viability evaluation of Fe3O4-NPs performed by using the MTT assay on the WEHI164 cells demonstrated no significant toxicity in higher concentration up to 140.0 ppm, which allows them to be used in some biological applications such as drug delivery. Conclusion: The presented synthesis method can be used for the controlled synthesis of Fe3O4-NPs, which could be found to be important in applications in biotechnology, biosensor and biomedicine, magnetic resonance imaging and catalysis.
AB - Introduction: In the present research, we report a quick and green synthesis of magnetite nanoparticles (Fe3O4-NPs) in aqueous solution using ferric and ferrous chloride, with different percentages of natural honey (0.5%, 1.0%, 3.0% and 5.0% w/v) as the precursors, stabilizer, reducing and capping agent, respectively. The effect of the stabilizer on the magnetic properties and size of Fe3O4-NPs was also studied. Methods: The nanoparticles were characterized by X-ray diffraction (XRD) analysis, field emission scanning electron microscopy, energy dispersive X-ray fluorescence, transmission electron microscopy (TEM), vibrating sample magnetometry (VSM) and Fourier transform infrared spectroscopy. Results: The XRD analysis indicated the presence of pure Fe3O4-NPs while the TEM images indicated that the Fe3O4-NPs are spherical with a diameter range between 3.21 and 2.22 nm. The VSM study demonstrated that the magnetic properties were enhanced with the decrease in the percentage of honey. In vitro viability evaluation of Fe3O4-NPs performed by using the MTT assay on the WEHI164 cells demonstrated no significant toxicity in higher concentration up to 140.0 ppm, which allows them to be used in some biological applications such as drug delivery. Conclusion: The presented synthesis method can be used for the controlled synthesis of Fe3O4-NPs, which could be found to be important in applications in biotechnology, biosensor and biomedicine, magnetic resonance imaging and catalysis.
KW - Fe3O4 nanoparticles
KW - green synthesis
KW - honey
KW - in vitro
KW - magnetic properties
KW - MTT assay
KW - transmission electron microscopy
KW - viability
KW - WEHI164 cells
UR - http://www.scopus.com/inward/record.url?scp=85057556732&partnerID=8YFLogxK
U2 - 10.2147/IJN.S158083
DO - 10.2147/IJN.S158083
M3 - Article
C2 - 30498350
AN - SCOPUS:85057556732
VL - 13
SP - 6903
EP - 6911
JO - International Journal of Nanomedicine
JF - International Journal of Nanomedicine
SN - 1178-2013
ER -