Use of dried blood spots to define antibody response to the Strongyloides stercoralis recombinant antigen NIE

Kate Mounsey, Therese Kearns, Melanie Rampton, Stacey Llewellyn, Mallory King, Deborah Holt, Bart Currie, Ross Andrews, Thomas Nutman, James McCarthy

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    An approach to improve the diagnosis of Strongyloides stercoralis infection is the use of serologic assays utilising the NIE antigen from S. stercoralis, with good diagnostic sensitivity and excellent specificity reported. Detection of antibody eluted from dried blood spots (DBS) has shown utility in large-scale seroepidemiological studies for a range of conditions and is appealing for use with children where sample collection is difficult. We adapted an existing NIE-enzyme linked immunosorbent assay (ELISA) for the testing of strongyloides antibody response on DBS, and evaluated it in a population screening and mass drug administration programme (MDA) for strongyloidiasis conducted in an Australian indigenous community. Study participants were treated with 200. ?g/kg ivermectin (>15. kg) or 3�400. mg albendazole (<15. kg). The sensitivity of the NIE DBS-ELISA was determined by receiver operator characteristic (ROC) analysis to be 85.7%. A total of 214 DBS were collected from 184 participants across two screening and MDA encounters. A total of 27 of 164 participants (16.5%) tested positive for S. stercoralis NIE-DBS prior to MDA treatment, and 6 of 50 participants (12.0%) tested positive after treatment. These prevalence values are similar to those documented by standard serology in the same community. For 30 participants where a DBS was collected at both MDA 1 and 2, a significant decline in ELISA values was evident post treatment (0.12-0.02, p= 0.0012). These results are in agreement with previous studies documenting the high seroprevalence of S. stercoralis in remote Australian Indigenous communities, and suggest that collection of dried blood spots may be a useful approach for field diagnosis of S. stercoralis seroprevalence. � 2014 Elsevier B.V.
    Original languageEnglish
    Pages (from-to)78-82
    Number of pages5
    JournalActa Tropica
    Volume138
    DOIs
    Publication statusPublished - 2014

    Fingerprint

    Strongyloides stercoralis
    recombinant antigens
    Antibody Formation
    Antigens
    antibodies
    blood
    Seroepidemiologic Studies
    drugs
    Preclinical Drug Evaluations
    Mass Screening
    Enzyme-Linked Immunosorbent Assay
    enzyme-linked immunosorbent assay
    seroprevalence
    strongyloidiasis
    Strongyloides
    Strongyloidiasis
    screening
    diagnostic sensitivity
    Albendazole
    Ivermectin

    Cite this

    Mounsey, Kate ; Kearns, Therese ; Rampton, Melanie ; Llewellyn, Stacey ; King, Mallory ; Holt, Deborah ; Currie, Bart ; Andrews, Ross ; Nutman, Thomas ; McCarthy, James. / Use of dried blood spots to define antibody response to the Strongyloides stercoralis recombinant antigen NIE. In: Acta Tropica. 2014 ; Vol. 138. pp. 78-82.
    @article{83f2c8b15b5b4e159fdc84e792323761,
    title = "Use of dried blood spots to define antibody response to the Strongyloides stercoralis recombinant antigen NIE",
    abstract = "An approach to improve the diagnosis of Strongyloides stercoralis infection is the use of serologic assays utilising the NIE antigen from S. stercoralis, with good diagnostic sensitivity and excellent specificity reported. Detection of antibody eluted from dried blood spots (DBS) has shown utility in large-scale seroepidemiological studies for a range of conditions and is appealing for use with children where sample collection is difficult. We adapted an existing NIE-enzyme linked immunosorbent assay (ELISA) for the testing of strongyloides antibody response on DBS, and evaluated it in a population screening and mass drug administration programme (MDA) for strongyloidiasis conducted in an Australian indigenous community. Study participants were treated with 200. ?g/kg ivermectin (>15. kg) or 3�400. mg albendazole (<15. kg). The sensitivity of the NIE DBS-ELISA was determined by receiver operator characteristic (ROC) analysis to be 85.7{\%}. A total of 214 DBS were collected from 184 participants across two screening and MDA encounters. A total of 27 of 164 participants (16.5{\%}) tested positive for S. stercoralis NIE-DBS prior to MDA treatment, and 6 of 50 participants (12.0{\%}) tested positive after treatment. These prevalence values are similar to those documented by standard serology in the same community. For 30 participants where a DBS was collected at both MDA 1 and 2, a significant decline in ELISA values was evident post treatment (0.12-0.02, p= 0.0012). These results are in agreement with previous studies documenting the high seroprevalence of S. stercoralis in remote Australian Indigenous communities, and suggest that collection of dried blood spots may be a useful approach for field diagnosis of S. stercoralis seroprevalence. � 2014 Elsevier B.V.",
    keywords = "albendazole, ivermectin, parasite antigen, permethrin, recombinant antigen, anthelmintic agent, helminth antibody, recombinant protein, antibody, antigen, epidemiology, immune response, indigenous population, nematode, parasite, serum, antibody detection, antibody response, article, Australia, clinical article, controlled study, diagnostic test accuracy study, diagnostic value, dried blood spot testing, enzyme linked immunosorbent assay, human, indigenous people, mass screening, prevalence, receiver operating characteristic, repeated drug dose, sensitivity and specificity, seroepidemiology, serology, single drug dose, Strongyloides stercoralis, strongyloidiasis, animal, blood, child, desiccation, diagnostic use, drug monitoring, evaluation study, female, immunology, laboratory diagnosis, male, population group, preschool child, procedures, Strongyloides, Vermes, Albendazole, Animals, Anthelmintics, Antibodies, Helminth, Antigens, Helminth, Child, Child, Preschool, Desiccation, Drug Monitoring, Enzyme-Linked Immunosorbent Assay, Female, Humans, Ivermectin, Male, Population Groups, Recombinant Proteins, ROC Curve, Sensitivity and Specificity, Seroepidemiologic Studies, Specimen Handling, Strongyloidiasis",
    author = "Kate Mounsey and Therese Kearns and Melanie Rampton and Stacey Llewellyn and Mallory King and Deborah Holt and Bart Currie and Ross Andrews and Thomas Nutman and James McCarthy",
    note = "NHMRC Grant no. 605804",
    year = "2014",
    doi = "10.1016/j.actatropica.2014.07.007",
    language = "English",
    volume = "138",
    pages = "78--82",
    journal = "Acta Tropica",
    issn = "0001-706X",
    publisher = "Elesvier BV",

    }

    Use of dried blood spots to define antibody response to the Strongyloides stercoralis recombinant antigen NIE. / Mounsey, Kate; Kearns, Therese; Rampton, Melanie; Llewellyn, Stacey; King, Mallory; Holt, Deborah; Currie, Bart; Andrews, Ross; Nutman, Thomas; McCarthy, James.

    In: Acta Tropica, Vol. 138, 2014, p. 78-82.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Use of dried blood spots to define antibody response to the Strongyloides stercoralis recombinant antigen NIE

    AU - Mounsey, Kate

    AU - Kearns, Therese

    AU - Rampton, Melanie

    AU - Llewellyn, Stacey

    AU - King, Mallory

    AU - Holt, Deborah

    AU - Currie, Bart

    AU - Andrews, Ross

    AU - Nutman, Thomas

    AU - McCarthy, James

    N1 - NHMRC Grant no. 605804

    PY - 2014

    Y1 - 2014

    N2 - An approach to improve the diagnosis of Strongyloides stercoralis infection is the use of serologic assays utilising the NIE antigen from S. stercoralis, with good diagnostic sensitivity and excellent specificity reported. Detection of antibody eluted from dried blood spots (DBS) has shown utility in large-scale seroepidemiological studies for a range of conditions and is appealing for use with children where sample collection is difficult. We adapted an existing NIE-enzyme linked immunosorbent assay (ELISA) for the testing of strongyloides antibody response on DBS, and evaluated it in a population screening and mass drug administration programme (MDA) for strongyloidiasis conducted in an Australian indigenous community. Study participants were treated with 200. ?g/kg ivermectin (>15. kg) or 3�400. mg albendazole (<15. kg). The sensitivity of the NIE DBS-ELISA was determined by receiver operator characteristic (ROC) analysis to be 85.7%. A total of 214 DBS were collected from 184 participants across two screening and MDA encounters. A total of 27 of 164 participants (16.5%) tested positive for S. stercoralis NIE-DBS prior to MDA treatment, and 6 of 50 participants (12.0%) tested positive after treatment. These prevalence values are similar to those documented by standard serology in the same community. For 30 participants where a DBS was collected at both MDA 1 and 2, a significant decline in ELISA values was evident post treatment (0.12-0.02, p= 0.0012). These results are in agreement with previous studies documenting the high seroprevalence of S. stercoralis in remote Australian Indigenous communities, and suggest that collection of dried blood spots may be a useful approach for field diagnosis of S. stercoralis seroprevalence. � 2014 Elsevier B.V.

    AB - An approach to improve the diagnosis of Strongyloides stercoralis infection is the use of serologic assays utilising the NIE antigen from S. stercoralis, with good diagnostic sensitivity and excellent specificity reported. Detection of antibody eluted from dried blood spots (DBS) has shown utility in large-scale seroepidemiological studies for a range of conditions and is appealing for use with children where sample collection is difficult. We adapted an existing NIE-enzyme linked immunosorbent assay (ELISA) for the testing of strongyloides antibody response on DBS, and evaluated it in a population screening and mass drug administration programme (MDA) for strongyloidiasis conducted in an Australian indigenous community. Study participants were treated with 200. ?g/kg ivermectin (>15. kg) or 3�400. mg albendazole (<15. kg). The sensitivity of the NIE DBS-ELISA was determined by receiver operator characteristic (ROC) analysis to be 85.7%. A total of 214 DBS were collected from 184 participants across two screening and MDA encounters. A total of 27 of 164 participants (16.5%) tested positive for S. stercoralis NIE-DBS prior to MDA treatment, and 6 of 50 participants (12.0%) tested positive after treatment. These prevalence values are similar to those documented by standard serology in the same community. For 30 participants where a DBS was collected at both MDA 1 and 2, a significant decline in ELISA values was evident post treatment (0.12-0.02, p= 0.0012). These results are in agreement with previous studies documenting the high seroprevalence of S. stercoralis in remote Australian Indigenous communities, and suggest that collection of dried blood spots may be a useful approach for field diagnosis of S. stercoralis seroprevalence. � 2014 Elsevier B.V.

    KW - albendazole

    KW - ivermectin

    KW - parasite antigen

    KW - permethrin

    KW - recombinant antigen

    KW - anthelmintic agent

    KW - helminth antibody

    KW - recombinant protein

    KW - antibody

    KW - antigen

    KW - epidemiology

    KW - immune response

    KW - indigenous population

    KW - nematode

    KW - parasite

    KW - serum

    KW - antibody detection

    KW - antibody response

    KW - article

    KW - Australia

    KW - clinical article

    KW - controlled study

    KW - diagnostic test accuracy study

    KW - diagnostic value

    KW - dried blood spot testing

    KW - enzyme linked immunosorbent assay

    KW - human

    KW - indigenous people

    KW - mass screening

    KW - prevalence

    KW - receiver operating characteristic

    KW - repeated drug dose

    KW - sensitivity and specificity

    KW - seroepidemiology

    KW - serology

    KW - single drug dose

    KW - Strongyloides stercoralis

    KW - strongyloidiasis

    KW - animal

    KW - blood

    KW - child

    KW - desiccation

    KW - diagnostic use

    KW - drug monitoring

    KW - evaluation study

    KW - female

    KW - immunology

    KW - laboratory diagnosis

    KW - male

    KW - population group

    KW - preschool child

    KW - procedures

    KW - Strongyloides

    KW - Vermes

    KW - Albendazole

    KW - Animals

    KW - Anthelmintics

    KW - Antibodies, Helminth

    KW - Antigens, Helminth

    KW - Child

    KW - Child, Preschool

    KW - Desiccation

    KW - Drug Monitoring

    KW - Enzyme-Linked Immunosorbent Assay

    KW - Female

    KW - Humans

    KW - Ivermectin

    KW - Male

    KW - Population Groups

    KW - Recombinant Proteins

    KW - ROC Curve

    KW - Sensitivity and Specificity

    KW - Seroepidemiologic Studies

    KW - Specimen Handling

    KW - Strongyloidiasis

    UR - http://www.scopus.com/inward/record.url?scp=84907595266&partnerID=8YFLogxK

    U2 - 10.1016/j.actatropica.2014.07.007

    DO - 10.1016/j.actatropica.2014.07.007

    M3 - Article

    VL - 138

    SP - 78

    EP - 82

    JO - Acta Tropica

    JF - Acta Tropica

    SN - 0001-706X

    ER -