Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection

Cassandra Faux, Katherine E Arden, Stephen B Lambert, Michael D Nissen, Terry Nolan, Anne Chang, Theo P Sloots, Ian M Mackay

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    Abstract

    We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)–specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.
    Original languageEnglish
    Pages (from-to)296-298
    Number of pages3
    JournalEmerging Infectious Diseases
    Volume17
    Issue number2
    DOIs
    Publication statusPublished - 2011

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    Faux, C., Arden, K. E., Lambert, S. B., Nissen, M. D., Nolan, T., Chang, A., ... Mackay, I. M. (2011). Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection. Emerging Infectious Diseases, 17(2), 296-298. https://doi.org/10.3201/eid1702.101123