We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)–specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.
Faux, C., Arden, K. E., Lambert, S. B., Nissen, M. D., Nolan, T., Chang, A., ... Mackay, I. M. (2011). Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection. Emerging Infectious Diseases, 17(2), 296-298. https://doi.org/10.3201/eid1702.101123