Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection

Cassandra Faux, Katherine E Arden, Stephen B Lambert, Michael D Nissen, Terry Nolan, Anne Chang, Theo P Sloots, Ian M Mackay

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Abstract

We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)–specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.
Original languageEnglish
Pages (from-to)296-298
Number of pages3
JournalEmerging Infectious Diseases
Volume17
Issue number2
DOIs
Publication statusPublished - 2011

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Rhinovirus
Polymerase Chain Reaction
Infection
Oligonucleotides
Epidemiology
Temperature

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Faux, C., Arden, K. E., Lambert, S. B., Nissen, M. D., Nolan, T., Chang, A., ... Mackay, I. M. (2011). Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection. Emerging Infectious Diseases, 17(2), 296-298. https://doi.org/10.3201/eid1702.101123
Faux, Cassandra ; Arden, Katherine E ; Lambert, Stephen B ; Nissen, Michael D ; Nolan, Terry ; Chang, Anne ; Sloots, Theo P ; Mackay, Ian M. / Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection. In: Emerging Infectious Diseases. 2011 ; Vol. 17, No. 2. pp. 296-298.
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Faux, C, Arden, KE, Lambert, SB, Nissen, MD, Nolan, T, Chang, A, Sloots, TP & Mackay, IM 2011, 'Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection', Emerging Infectious Diseases, vol. 17, no. 2, pp. 296-298. https://doi.org/10.3201/eid1702.101123

Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection. / Faux, Cassandra; Arden, Katherine E; Lambert, Stephen B; Nissen, Michael D; Nolan, Terry; Chang, Anne; Sloots, Theo P; Mackay, Ian M.

In: Emerging Infectious Diseases, Vol. 17, No. 2, 2011, p. 296-298.

Research output: Contribution to journalArticleResearchpeer-review

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