Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection

Cassandra Faux; Katherine E Arden; Stephen B Lambert; Michael D Nissen; Terry Nolan; Anne Chang; Theo P Sloots; Ian M Mackay

doi:10.3201/eid1702.101123

Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection

Cassandra Faux, Katherine E Arden, Stephen B Lambert, Michael D Nissen, Terry Nolan, Anne Chang, Theo P Sloots, Ian M Mackay

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Abstract

We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)–specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.

Original language	English
Pages (from-to)	296-298
Number of pages	3
Journal	Emerging Infectious Diseases
Volume	17
Issue number	2
DOIs	https://doi.org/10.3201/eid1702.101123
Publication status	Published - 2011

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5326203-oaFinal published version, 251 KBLicence: CC BY

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title = "Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection",

abstract = "We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)–specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.",

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AU - Faux, Cassandra

AU - Arden, Katherine E

AU - Lambert, Stephen B

AU - Nissen, Michael D

AU - Nolan, Terry

AU - Chang, Anne

AU - Sloots, Theo P

AU - Mackay, Ian M

PY - 2011

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AB - We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)–specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.

U2 - 10.3201/eid1702.101123

DO - 10.3201/eid1702.101123

M3 - Article

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EP - 298

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

SN - 1080-6040

IS - 2

ER -

Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection

Abstract

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