Using BOX-PCR to exclude a clonal outbreak of melioidosis

Bart Currie, D Gal, Mark Mayo, Linda Ward, Daniel Godoy, Brian Spratt, John LiPuma

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    Background: Although melioidosis in endemic regions is usually caused by a diverse range of Burkholderia pseudomallei strains, clonal outbreaks from contaminated potable water have been described. Furthermore B. pseudomallei is classified as a CDC Group B bioterrorism agent. Ribotyping, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) have been used to identify genetically related B. pseudomallei isolates, but they are time consuming and technically challenging for many laboratories. Methods: We have adapted repetitive sequence typing using a BOX A1R primer for typing B. pseudomallei and compared BOX-PCR fingerprinting results on a wide range of well-characterized B. pseudomallei isolates with MLST and PFGE performed on the same isolates. Results: BOX-PCR typing compared favourably with MLST and PFGE performed on the same isolates, both discriminating between the majority of multilocus sequence types and showing relatedness between epidemiologically linked isolates from various outbreak clusters. Conclusion: Our results suggest that BOX-PCR can be used to exclude a clonal outbreak of melioidosis within 10 hours of receiving the bacterial strains. � 2007 Currie et al; licensee BioMed Central Ltd.
    Original languageEnglish
    Pages (from-to)68-
    JournalBMC Infectious Diseases
    Volume7
    Publication statusPublished - 2007

    Fingerprint

    Melioidosis
    Burkholderia pseudomallei
    Disease Outbreaks
    Multilocus Sequence Typing
    Pulsed Field Gel Electrophoresis
    Polymerase Chain Reaction
    Biological Warfare Agents
    Ribotyping
    Nucleic Acid Repetitive Sequences
    Centers for Disease Control and Prevention (U.S.)
    Drinking Water

    Cite this

    Currie, Bart ; Gal, D ; Mayo, Mark ; Ward, Linda ; Godoy, Daniel ; Spratt, Brian ; LiPuma, John. / Using BOX-PCR to exclude a clonal outbreak of melioidosis. In: BMC Infectious Diseases. 2007 ; Vol. 7. pp. 68-.
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    abstract = "Background: Although melioidosis in endemic regions is usually caused by a diverse range of Burkholderia pseudomallei strains, clonal outbreaks from contaminated potable water have been described. Furthermore B. pseudomallei is classified as a CDC Group B bioterrorism agent. Ribotyping, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) have been used to identify genetically related B. pseudomallei isolates, but they are time consuming and technically challenging for many laboratories. Methods: We have adapted repetitive sequence typing using a BOX A1R primer for typing B. pseudomallei and compared BOX-PCR fingerprinting results on a wide range of well-characterized B. pseudomallei isolates with MLST and PFGE performed on the same isolates. Results: BOX-PCR typing compared favourably with MLST and PFGE performed on the same isolates, both discriminating between the majority of multilocus sequence types and showing relatedness between epidemiologically linked isolates from various outbreak clusters. Conclusion: Our results suggest that BOX-PCR can be used to exclude a clonal outbreak of melioidosis within 10 hours of receiving the bacterial strains. � 2007 Currie et al; licensee BioMed Central Ltd.",
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    Using BOX-PCR to exclude a clonal outbreak of melioidosis. / Currie, Bart; Gal, D; Mayo, Mark; Ward, Linda; Godoy, Daniel; Spratt, Brian; LiPuma, John.

    In: BMC Infectious Diseases, Vol. 7, 2007, p. 68-.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Using BOX-PCR to exclude a clonal outbreak of melioidosis

    AU - Currie, Bart

    AU - Gal, D

    AU - Mayo, Mark

    AU - Ward, Linda

    AU - Godoy, Daniel

    AU - Spratt, Brian

    AU - LiPuma, John

    PY - 2007

    Y1 - 2007

    N2 - Background: Although melioidosis in endemic regions is usually caused by a diverse range of Burkholderia pseudomallei strains, clonal outbreaks from contaminated potable water have been described. Furthermore B. pseudomallei is classified as a CDC Group B bioterrorism agent. Ribotyping, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) have been used to identify genetically related B. pseudomallei isolates, but they are time consuming and technically challenging for many laboratories. Methods: We have adapted repetitive sequence typing using a BOX A1R primer for typing B. pseudomallei and compared BOX-PCR fingerprinting results on a wide range of well-characterized B. pseudomallei isolates with MLST and PFGE performed on the same isolates. Results: BOX-PCR typing compared favourably with MLST and PFGE performed on the same isolates, both discriminating between the majority of multilocus sequence types and showing relatedness between epidemiologically linked isolates from various outbreak clusters. Conclusion: Our results suggest that BOX-PCR can be used to exclude a clonal outbreak of melioidosis within 10 hours of receiving the bacterial strains. � 2007 Currie et al; licensee BioMed Central Ltd.

    AB - Background: Although melioidosis in endemic regions is usually caused by a diverse range of Burkholderia pseudomallei strains, clonal outbreaks from contaminated potable water have been described. Furthermore B. pseudomallei is classified as a CDC Group B bioterrorism agent. Ribotyping, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) have been used to identify genetically related B. pseudomallei isolates, but they are time consuming and technically challenging for many laboratories. Methods: We have adapted repetitive sequence typing using a BOX A1R primer for typing B. pseudomallei and compared BOX-PCR fingerprinting results on a wide range of well-characterized B. pseudomallei isolates with MLST and PFGE performed on the same isolates. Results: BOX-PCR typing compared favourably with MLST and PFGE performed on the same isolates, both discriminating between the majority of multilocus sequence types and showing relatedness between epidemiologically linked isolates from various outbreak clusters. Conclusion: Our results suggest that BOX-PCR can be used to exclude a clonal outbreak of melioidosis within 10 hours of receiving the bacterial strains. � 2007 Currie et al; licensee BioMed Central Ltd.

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