TY - CHAP
T1 - Whole Blood Dendritic Cell Cytokine Production Assay
AU - Woodberry, Tonia
AU - Loughland, Jessica Rita
AU - Minigo, Gabriela
N1 - Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022/7/27
Y1 - 2022/7/27
N2 - This protocol outlines a method for the timely detection of intracellular cytokines produced by activated dendritic cells (DC) in human whole blood. The quantification of cytokines is used to measure DC immune responsiveness, providing information on the breadth, strength, and DC subtypes responding spontaneously and to specific stimulation with toll-like receptor (TLR) ligands or parasite-infected erythrocytes. DC subsets, plasmacytoid DC, CD1c+ DC, CD141+ DC, and CD16+ DC, are examined in their natural environment of plasma and blood cells (erythrocytes, neutrophils, platelets, and leukocytes) enabling disease, medication, nutritional, and hematological effects on DC function to be examined in vaccine studies, ageing, health, and disease.
AB - This protocol outlines a method for the timely detection of intracellular cytokines produced by activated dendritic cells (DC) in human whole blood. The quantification of cytokines is used to measure DC immune responsiveness, providing information on the breadth, strength, and DC subtypes responding spontaneously and to specific stimulation with toll-like receptor (TLR) ligands or parasite-infected erythrocytes. DC subsets, plasmacytoid DC, CD1c+ DC, CD141+ DC, and CD16+ DC, are examined in their natural environment of plasma and blood cells (erythrocytes, neutrophils, platelets, and leukocytes) enabling disease, medication, nutritional, and hematological effects on DC function to be examined in vaccine studies, ageing, health, and disease.
KW - Blood dendritic cell antigen (BDCA)
KW - Dendritic cell subsets
KW - Human assay
KW - Intracellular cytokine production
KW - Malaria
KW - Parasite
KW - Toll-like receptor
KW - Whole blood
UR - http://www.scopus.com/inward/record.url?scp=85135383444&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-2189-9_53
DO - 10.1007/978-1-0716-2189-9_53
M3 - Chapter
C2 - 35881385
AN - SCOPUS:85135383444
SN - 978-1-0716-2188-2
T3 - Methods in Molecular Biology
SP - 715
EP - 729
BT - Malaria Immunology
A2 - Jensen, Anja Tatiana Ramstedt
A2 - Hviid, Lars
PB - Humana Press Inc.
CY - New York
ER -