Abstract
This protocol outlines a method for the timely detection of intracellular cytokines produced by activated dendritic cells (DC) in human whole blood. The quantification of cytokines is used to measure DC immune responsiveness, providing information on the breadth, strength, and DC subtypes responding spontaneously and to specific stimulation with toll-like receptor (TLR) ligands or parasite-infected erythrocytes. DC subsets, plasmacytoid DC, CD1c+ DC, CD141+ DC, and CD16+ DC, are examined in their natural environment of plasma and blood cells (erythrocytes, neutrophils, platelets, and leukocytes) enabling disease, medication, nutritional, and hematological effects on DC function to be examined in vaccine studies, ageing, health, and disease.
Original language | English |
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Title of host publication | Malaria Immunology |
Subtitle of host publication | Targeting the Surface of Infected Erythrocytes |
Editors | Anja Tatiana Ramstedt Jensen, Lars Hviid |
Place of Publication | New York |
Publisher | Humana Press Inc. |
Pages | 715-729 |
Number of pages | 15 |
Edition | 1 |
ISBN (Electronic) | 978-1-0716-2189-9 |
ISBN (Print) | 978-1-0716-2188-2 |
DOIs | |
Publication status | Published - 27 Jul 2022 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 2470 |
ISSN (Print) | 1064-3745 |
ISSN (Electronic) | 1940-6029 |
Bibliographical note
Publisher Copyright:© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.