Whole Blood Dendritic Cell Cytokine Production Assay

Tonia Woodberry; Jessica Rita Loughland; Gabriela Minigo

doi:10.1007/978-1-0716-2189-9_53

Whole Blood Dendritic Cell Cytokine Production Assay

Tonia Woodberry, Jessica Rita Loughland, Gabriela Minigo

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

This protocol outlines a method for the timely detection of intracellular cytokines produced by activated dendritic cells (DC) in human whole blood. The quantification of cytokines is used to measure DC immune responsiveness, providing information on the breadth, strength, and DC subtypes responding spontaneously and to specific stimulation with toll-like receptor (TLR) ligands or parasite-infected erythrocytes. DC subsets, plasmacytoid DC, CD1c⁺ DC, CD141⁺ DC, and CD16⁺ DC, are examined in their natural environment of plasma and blood cells (erythrocytes, neutrophils, platelets, and leukocytes) enabling disease, medication, nutritional, and hematological effects on DC function to be examined in vaccine studies, ageing, health, and disease.

Original language	English
Title of host publication	Malaria Immunology
Subtitle of host publication	Targeting the Surface of Infected Erythrocytes
Editors	Anja Tatiana Ramstedt Jensen, Lars Hviid
Place of Publication	New York
Publisher	Humana Press Inc.
Pages	715-729
Number of pages	15
Edition	1
ISBN (Electronic)	978-1-0716-2189-9
ISBN (Print)	978-1-0716-2188-2
DOIs	https://doi.org/10.1007/978-1-0716-2189-9_53
Publication status	Published - 27 Jul 2022

Publication series

Name	Methods in Molecular Biology
Volume	2470
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

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10.1007/978-1-0716-2189-9_53

Cite this

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title = "Whole Blood Dendritic Cell Cytokine Production Assay",

abstract = "This protocol outlines a method for the timely detection of intracellular cytokines produced by activated dendritic cells (DC) in human whole blood. The quantification of cytokines is used to measure DC immune responsiveness, providing information on the breadth, strength, and DC subtypes responding spontaneously and to specific stimulation with toll-like receptor (TLR) ligands or parasite-infected erythrocytes. DC subsets, plasmacytoid DC, CD1c+ DC, CD141+ DC, and CD16+ DC, are examined in their natural environment of plasma and blood cells (erythrocytes, neutrophils, platelets, and leukocytes) enabling disease, medication, nutritional, and hematological effects on DC function to be examined in vaccine studies, ageing, health, and disease.",

keywords = "Blood dendritic cell antigen (BDCA), Dendritic cell subsets, Human assay, Intracellular cytokine production, Malaria, Parasite, Toll-like receptor, Whole blood",

author = "Tonia Woodberry and Loughland, {Jessica Rita} and Gabriela Minigo",

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Whole Blood Dendritic Cell Cytokine Production Assay. / Woodberry, Tonia; Loughland, Jessica Rita; Minigo, Gabriela.

Malaria Immunology: Targeting the Surface of Infected Erythrocytes. ed. / Anja Tatiana Ramstedt Jensen; Lars Hviid. 1. ed. New York : Humana Press Inc., 2022. p. 715-729 (Methods in Molecular Biology; Vol. 2470).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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AU - Loughland, Jessica Rita

AU - Minigo, Gabriela

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N2 - This protocol outlines a method for the timely detection of intracellular cytokines produced by activated dendritic cells (DC) in human whole blood. The quantification of cytokines is used to measure DC immune responsiveness, providing information on the breadth, strength, and DC subtypes responding spontaneously and to specific stimulation with toll-like receptor (TLR) ligands or parasite-infected erythrocytes. DC subsets, plasmacytoid DC, CD1c+ DC, CD141+ DC, and CD16+ DC, are examined in their natural environment of plasma and blood cells (erythrocytes, neutrophils, platelets, and leukocytes) enabling disease, medication, nutritional, and hematological effects on DC function to be examined in vaccine studies, ageing, health, and disease.

AB - This protocol outlines a method for the timely detection of intracellular cytokines produced by activated dendritic cells (DC) in human whole blood. The quantification of cytokines is used to measure DC immune responsiveness, providing information on the breadth, strength, and DC subtypes responding spontaneously and to specific stimulation with toll-like receptor (TLR) ligands or parasite-infected erythrocytes. DC subsets, plasmacytoid DC, CD1c+ DC, CD141+ DC, and CD16+ DC, are examined in their natural environment of plasma and blood cells (erythrocytes, neutrophils, platelets, and leukocytes) enabling disease, medication, nutritional, and hematological effects on DC function to be examined in vaccine studies, ageing, health, and disease.

KW - Blood dendritic cell antigen (BDCA)

KW - Dendritic cell subsets

KW - Human assay

KW - Intracellular cytokine production

KW - Malaria

KW - Parasite

KW - Toll-like receptor

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SN - 978-1-0716-2188-2

T3 - Methods in Molecular Biology

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BT - Malaria Immunology

A2 - Jensen, Anja Tatiana Ramstedt

A2 - Hviid, Lars

PB - Humana Press Inc.

CY - New York

ER -

Whole Blood Dendritic Cell Cytokine Production Assay

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