Whole Blood Dendritic Cell Cytokine Production Assay

Tonia Woodberry, Jessica Rita Loughland, Gabriela Minigo

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

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Abstract

This protocol outlines a method for the timely detection of intracellular cytokines produced by activated dendritic cells (DC) in human whole blood. The quantification of cytokines is used to measure DC immune responsiveness, providing information on the breadth, strength, and DC subtypes responding spontaneously and to specific stimulation with toll-like receptor (TLR) ligands or parasite-infected erythrocytes. DC subsets, plasmacytoid DC, CD1c+ DC, CD141+ DC, and CD16+ DC, are examined in their natural environment of plasma and blood cells (erythrocytes, neutrophils, platelets, and leukocytes) enabling disease, medication, nutritional, and hematological effects on DC function to be examined in vaccine studies, ageing, health, and disease.

Original languageEnglish
Title of host publicationMalaria Immunology
Subtitle of host publicationTargeting the Surface of Infected Erythrocytes
EditorsAnja Tatiana Ramstedt Jensen, Lars Hviid
Place of PublicationNew York
PublisherHumana Press Inc.
Pages715-729
Number of pages15
Edition1
ISBN (Electronic)978-1-0716-2189-9
ISBN (Print)978-1-0716-2188-2
DOIs
Publication statusPublished - 27 Jul 2022

Publication series

NameMethods in Molecular Biology
Volume2470
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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