Whole-genome characterization and genotyping of global WU polyomavirus strains

Seweryn Bialasiewicz, Rebecca Rockett, David W Whiley, Yacine Abed, Tobias Allander, Michael Binks, Guy Boivin, Allen Cheng, Ju-Young Chung, Patricia E Ferguson, Nicole M Gilroy, Amanda Leach, Cecilia Lindau, John W Rossen, Tania C Sorrell, Michael D Nissen, Theo P Sloots

    Research output: Contribution to journalArticlepeer-review


    Exploration of the genetic diversity of WU polyomavirus (WUV) has been limited in terms of the specimen numbers and particularly the sizes of the genomic fragments analyzed. Using whole-genome sequencing of 48 WUV strains collected in four continents over a 5-year period and 16 publicly available whole-genome sequences, we identified three main WUV clades and five subtypes, provisionally termed Ia, Ib, Ic, II, IIIa, and IIIb. Overall nucleotide variation was low (0 to 1.2%). The discriminatory power of the previous VP2 fragment typing method was found to be limited, and a new, larger genotyping region within the VP2/1 interface was proposed. Copyright � 2010, American Society for Microbiology. All Rights Reserved.
    Original languageEnglish
    Pages (from-to)6229-6234
    Number of pages6
    JournalJournal of Virology
    Issue number12
    Publication statusPublished - 2010

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