Whole genome sequencing reveals extensive community-level transmission of group A Streptococcus in remote communities

Asha Bowen, Tegan Harris, Deborah Holt, Philip Giffard, Jonathan Carapetis, Patricia Therese Herese Campbell, J McVernon, Steven Tong

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    Impetigo is common in remote Indigenous children of northern Australia, with the primary driver in this context being Streptococcus pyogenes [or group A Streptococcus (GAS)]. To reduce the high burden of impetigo, the transmission dynamics of GAS must be more clearly elucidated. We performed whole genome sequencing on 31 GAS isolates collected in a single community from children in 11 households with ⩾2 GAS-infected children. We aimed to determine whether transmission was occurring principally within households or across the community. The 31 isolates were represented by nine multilocus sequence types and isolates within each sequence type differed from one another by only 0–3 single nucleotide polymorphisms. There was evidence of extensive transmission both within households and across the community. Our findings suggest that strategies to reduce the burden of impetigo in this setting will need to extend beyond individual households, and incorporate multi-faceted, community-wide approaches.
    Original languageEnglish
    Pages (from-to)1991-1998
    Number of pages8
    JournalEpidemiology and Infection
    Volume144
    Issue number9
    DOIs
    Publication statusPublished - Jul 2016

    Fingerprint

    Impetigo
    Streptococcus
    Genome
    Streptococcus pyogenes
    Single Nucleotide Polymorphism

    Cite this

    Bowen, Asha ; Harris, Tegan ; Holt, Deborah ; Giffard, Philip ; Carapetis, Jonathan ; Campbell, Patricia Therese Herese ; McVernon, J ; Tong, Steven. / Whole genome sequencing reveals extensive community-level transmission of group A Streptococcus in remote communities. In: Epidemiology and Infection. 2016 ; Vol. 144, No. 9. pp. 1991-1998.
    @article{b0b39bbbc3a545edacc316edf4a355f1,
    title = "Whole genome sequencing reveals extensive community-level transmission of group A Streptococcus in remote communities",
    abstract = "Impetigo is common in remote Indigenous children of northern Australia, with the primary driver in this context being Streptococcus pyogenes [or group A Streptococcus (GAS)]. To reduce the high burden of impetigo, the transmission dynamics of GAS must be more clearly elucidated. We performed whole genome sequencing on 31 GAS isolates collected in a single community from children in 11 households with ⩾2 GAS-infected children. We aimed to determine whether transmission was occurring principally within households or across the community. The 31 isolates were represented by nine multilocus sequence types and isolates within each sequence type differed from one another by only 0–3 single nucleotide polymorphisms. There was evidence of extensive transmission both within households and across the community. Our findings suggest that strategies to reduce the burden of impetigo in this setting will need to extend beyond individual households, and incorporate multi-faceted, community-wide approaches.",
    author = "Asha Bowen and Tegan Harris and Deborah Holt and Philip Giffard and Jonathan Carapetis and Campbell, {Patricia Therese Herese} and J McVernon and Steven Tong",
    year = "2016",
    month = "7",
    doi = "10.1017/S095026881500326X",
    language = "English",
    volume = "144",
    pages = "1991--1998",
    journal = "Epidemiology and Infection",
    issn = "0950-2688",
    publisher = "Cambridge University Press",
    number = "9",

    }

    Whole genome sequencing reveals extensive community-level transmission of group A Streptococcus in remote communities. / Bowen, Asha; Harris, Tegan; Holt, Deborah; Giffard, Philip; Carapetis, Jonathan; Campbell, Patricia Therese Herese; McVernon, J; Tong, Steven.

    In: Epidemiology and Infection, Vol. 144, No. 9, 07.2016, p. 1991-1998.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Whole genome sequencing reveals extensive community-level transmission of group A Streptococcus in remote communities

    AU - Bowen, Asha

    AU - Harris, Tegan

    AU - Holt, Deborah

    AU - Giffard, Philip

    AU - Carapetis, Jonathan

    AU - Campbell, Patricia Therese Herese

    AU - McVernon, J

    AU - Tong, Steven

    PY - 2016/7

    Y1 - 2016/7

    N2 - Impetigo is common in remote Indigenous children of northern Australia, with the primary driver in this context being Streptococcus pyogenes [or group A Streptococcus (GAS)]. To reduce the high burden of impetigo, the transmission dynamics of GAS must be more clearly elucidated. We performed whole genome sequencing on 31 GAS isolates collected in a single community from children in 11 households with ⩾2 GAS-infected children. We aimed to determine whether transmission was occurring principally within households or across the community. The 31 isolates were represented by nine multilocus sequence types and isolates within each sequence type differed from one another by only 0–3 single nucleotide polymorphisms. There was evidence of extensive transmission both within households and across the community. Our findings suggest that strategies to reduce the burden of impetigo in this setting will need to extend beyond individual households, and incorporate multi-faceted, community-wide approaches.

    AB - Impetigo is common in remote Indigenous children of northern Australia, with the primary driver in this context being Streptococcus pyogenes [or group A Streptococcus (GAS)]. To reduce the high burden of impetigo, the transmission dynamics of GAS must be more clearly elucidated. We performed whole genome sequencing on 31 GAS isolates collected in a single community from children in 11 households with ⩾2 GAS-infected children. We aimed to determine whether transmission was occurring principally within households or across the community. The 31 isolates were represented by nine multilocus sequence types and isolates within each sequence type differed from one another by only 0–3 single nucleotide polymorphisms. There was evidence of extensive transmission both within households and across the community. Our findings suggest that strategies to reduce the burden of impetigo in this setting will need to extend beyond individual households, and incorporate multi-faceted, community-wide approaches.

    UR - http://www.scopus.com/inward/record.url?scp=84973138071&partnerID=8YFLogxK

    U2 - 10.1017/S095026881500326X

    DO - 10.1017/S095026881500326X

    M3 - Article

    VL - 144

    SP - 1991

    EP - 1998

    JO - Epidemiology and Infection

    JF - Epidemiology and Infection

    SN - 0950-2688

    IS - 9

    ER -